透明质酸复合BMP-2转染骨髓间充质干细胞修复兔桡骨干骨缺损  

Hyaluronic acid mixed with BMP-2 gene transfected bone marrow derived mesenchymal stem cells for repair of diaphyseal defects in rabbits

作　　者：章军辉[1] 陈永强[1] 汤亭亭[1] 楼觉人[1] 郝永强[1] 郁朝峰[1] 戴尅戎[1] 

机构地区：[1]上海第二医科大学附属第九人民医院骨科,浙江省宁波市第六医院骨科200011

出　　处：《中华骨科杂志》2005年第10期608-612,共5页Chinese Journal of Orthopaedics

基　　金：上海市科技发展基金国际合作项目(014307021)

摘　　要：目的评价携带人BMP-2(hBMP-2)基因重组腺病毒(Adv-hBMP-2)转染的骨髓间充质干细胞(BMSCs)与透明质酸(HA)复合构建的组织工程化骨对骨缺损的修复效果。方法取兔骨髓行BMSCs培养及Adv-hBMP-2的体外转染,建立桡骨干1.5cm缺损模型。20只兔(40侧)分四组(n=10):第一至三组分别注射Adv-hBMP-2转染细胞+HA、Adv-hBMP-2转染细胞、单纯HA,第四组为空白对照。结果(1)注射后第12周,第一组8侧骨缺损中6侧完全愈合,皮质骨形成,部分髓腔再通;第二组8侧骨缺损中有3侧完全愈合;第三、四组骨缺损均未愈合。X线疗效评分各组差异有统计学意义。(2)第一、二组4～8周时骨缺损内有多量新生骨痂形成,12周时部分髓腔再通;第三、四组4～8周时骨缺损处为纤维组织填充,12周时骨缺损未愈合,两骨端硬化。第一、二组之间新生骨小梁面积差异有统计学意义。(3)第一组的最大压缩载荷和弹性模量分别为(211.54±63.58)N和(113.36±56.47)MPa,第二组为(126.74±53.13)N和(98.91±63.36)MPa,差异有统计学意义。平均最大压缩载荷与正常桡骨之比:前者为75.86%,后者为45.45%。结论HA复合Adv-hBMP-2转染BMSCs构建的组织工程化骨可以修复兔桡骨干骨缺损,HA是一种安全的、有效的组织工程载体。Objective To evaluate osteogenetic effectiveness of hyaluronic acid （HA） mixed with adenovirus mediated human bone morphogenetic protein-2 gene （Adv-hBMP-2） transfeeted hone marrow derived mesenchvmal stem cells （BMSCs） of rabbits in the repair of radial shaft defects of rabbits. Methods The BMSCs were collected from bone marrow of rahbits, cuhured and transfected by Adv-hBMP-2 in vitro. The radial shaft defects （1.5 cm） models of 20 rabbits （40 sides） were created and then respectively injected the cells and/or materials according to the following four groups （n=10）： Ⅰ , Adv-hBMP-2 transfected BMSCs＋ HA; Ⅱ, Adv-hBMP-2 transfected BMSCs： Ⅲ, HA： Ⅳ. control. Results The bone defects of 6 out of 8 sides in the group I were repaired comptetely and partial bone marrow cavities were revasculized. Only 3 sides were repaired completely in the group Ⅱ and no side was repaired completely in the groups Ⅲ and control. There were statistic significance between each groups in X-ray scores. In the groups of Ⅰ and Ⅱ , mueh woven callus formed in the sites of the bone defects in the 4-Sth week after injection. Partial bone marrow cavities were revasculized in the 12th week after injection; while in the groups of HA and control, the sites of bone defects were mainly full of fibrous tissue and no healing happened. Both ends of the hone defects were hardened. There was significant difference statistically in the new trabeeular bone area in the groups of Ⅰ and Ⅱ . The maximunl vertical loading and elastic modulus were significant differenet in the group Ⅰ and Ⅱ . The ratios of average maximum vertical loading/normal value of radial shaft in the groups of Ⅰ and Ⅱ were 75.86% and 45.45%. respectively. Conclusion The tissue-engineered bone made by HA mixed with Adv-hBMP-2 transfected BMSCs can repair the bone defects in the radial shaft of rabbits. HA is a safe, effective carrier for tissue engineering.

关 键 词：骨形态发生蛋白类 基因疗法 间质干细胞 透明质酸 兔 骨 骨髓间充质干细胞 桡骨干骨缺损 干细胞修复 体外转染 透明质酸 BMP-2 复合 组织工程化骨 基因重组腺病毒 

分 类 号：R683.42[医药卫生—骨科学]