己酮可可碱对NOD小鼠1型糖尿病的免疫干预作用  

Immunointerventional effect of Pentoxifylline on diabetes in NOD mice

作　　者：施念玮[1] 吴艺捷[1] 王煜非[1] 顾丽萍[1] 

机构地区：[1]上海市第一人民医院内分泌科糖尿病研究室,200080

出　　处：《上海医学》2005年第10期877-880,F0003,共5页Shanghai Medical Journal

基　　金：上海市卫生局科技发展基金资助项目(01439)

摘　　要：目的观察己酮可可碱(PTX)对NOD小鼠1型糖尿病的免疫干预作用及其机制。方法采用NOD小鼠以环磷酰胺加速发病,给PTX后测血糖、糖尿病患病率,苏木精伊红染色及免疫组织化学法观察胰岛炎,采用半定量逆转录聚合酶链反应(RTPCR)分析胰腺组织γ干扰素(IFNγ)、肿瘤坏死因子α(TNFα)、白介素10(IL10)mRNA的表达。结果实验结束时,PTX组的血糖值为13.04mmol/L,明显低于对照组的20.53mmol/L(P<0.01);胰岛炎评分为1.02±0.98,明显低于对照组的2.27±1.22(P<0.05);糖尿病患病率为40.63%,明显低于对照组的71.43%(P<0.05);胰腺组织IFNγ、TNFαmRNA的表达降低(P<0.05),IL10mRNA的表达无明显改变。结论PTX可预防NOD小鼠糖尿病的发生,其机制可能与下调胰腺组织辅助性T细胞1型细胞因子有关。Objective To investigate the interventional effect of Pentoxifylline on type 1 diabetes mellitus in non-obese diabetic mice and to explore the possible mechanism. Methods 8-week-old NOD mice were treated with PTX to investigate the incidence of cyclophosphamide accelerated insulitis, and the expressions of IFN-γ, TNF-α, IL-10 mRNA in the pancreas detected by RT-PCR. Results After completion of this study, the mean blood glucose of the PTX group （13.04 mmol/L） was less than that of the control group（20.53 mmol/L, P〈0.01） ,the incidence of diabetes in the PTX group （40.63%）was reduced （P〈0.05）, The mean grade of in- sulitis was much lower in PTX group（1.02±0.98,P〈0.05）. The expression of cytokines mRNA in the PTX group being： TNF-α mRNA （0.46±0.32） ,IFN-γ mRNA （0.65 ±0.10）, which were lower than those of the control group （P〈0.05）, IL-10 mRNA expression （1.76±0.30） was unaffected as comparing with that of the control group （P〉0.05）. Concision PTX can prevent NOD mice from developing into type 1 diabetes, which may be related to suppression of Th1 cytokines. （Shanghai Med J, 2005,28 ： 877-880）

关 键 词：己酮可可碱 1型糖尿病 NOD鼠 细胞因子 预防 

分 类 号：R587.1[医药卫生—内分泌] R972[医药卫生—内科学]