氨态氮、亚硝态氮对罗氏沼虾血细胞及超微结构的影响  

Effects of ammonia-N and nitrite-N on the hemocyte count and ultrastructure of Macrobrachium rosenbergii

作　　者：胡义波[1] 王玥[1] 姜乃澄[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310028

出　　处：《浙江大学学报（理学版）》2005年第6期691-697,共7页Journal of Zhejiang University（Science Edition）

基　　金：浙江省九五重点资助项目(961102205)

摘　　要：氨态氮和亚硝态氮是虾类养殖环境中最主要的外来和自身污染物.在1 mg/L非离子氨态氮和1 mg/L亚硝态氮的作用条件下,罗氏沼虾总血细胞计数均有减少,且亚硝态氮的短期作用大于氨态氮;透射电镜观察结果表明,在氨态氮和亚硝态氮作用前后,罗氏沼虾血细胞超微结构发生改变,透明细胞、半颗粒细胞和颗粒细胞这三种血细胞的粗面内质网、核糖体和线粒体数量减少,其中以氨态氮作用引起的血细胞超微结构的变化幅度较大.这说明1 mg/L的非离子氨态氮和亚硝态氮对罗氏沼虾总血细胞计数和超微结构具有显著的影响.Ammonia-N and nitrite-N are the main pollutants in the shrimp culture environment. Exposure to 1 mg/L non-ionic ammonia-N and 1 mg/L nitrite-N, the total hemocyte count of Macrobrachium rosenbergii decreased significantly and the impact of nitrite-N was stronger. After exposure to ammonia-N and nitrite-N 10 days, the changes of hemocyte ultrastructures of M. rosenbergii were well observed. The quantities of rough endoplasmic reticulum, ribosome and mitochondrion were reduced and the ultrastructural alterations caused by ammonia-N were bigger. It was shown suggest that the total hemocyte counts and hemocyte ultrastructures of M. rosenbergii were affected significantly by exposure to 1 mg/L non-ionic ammonia-N and nitrite-N.

关 键 词：罗氏沼虾 氨态氮 亚硝态氮 血细胞 超微结构 

分 类 号：Q952.2[生物学—动物学] Q958.11