过氧化氢酶和黏附素保守区二价疫苗治疗小鼠幽门螺杆菌感染  

Immunzition treatment of Helicobacter pylori infection with two valence vaccine composed of catalase and adhesin conservation: An animal model study

作　　者：白杨[1] 陈丽[2] 王继德[1] 施理[1] 唱韶红[3] 张兆山[3] 周殿元[1] 张亚历[1] 

机构地区：[1]南方医科大学南方医院全军消化病研究所,广东广州510515 [2]江苏省苏北人民医院感染科,江苏扬州225002 [3]军事医学科学院生物工程研究所,北京100071

出　　处：《第四军医大学学报》2005年第21期1951-1953,共3页Journal of the Fourth Military Medical University

基　　金：863计划专题(102-07-03-06);国家自然科学基金(3017-0890);军队"十五"医药卫生科研课题(OIMA-132)

摘　　要：目的:利用人类幽门螺杆菌(H.pylori)感染的小鼠模型研究过氧化氢酶和黏附素保守区二价疫苗治疗H.pylori感染的作用.方法:把已感染H.pylori的小白鼠分成7组,分别通过灌胃方法给予过氧化氢酶和黏附素保守区(各100μg)加霍乱毒素CT(2μg)、过氧化氢酶(100μg)加CT(2μg)、黏附素保守区(100μg)加CT(2μg),PBS,单纯过氧化氢酶(100μg)、单纯黏附素保守区(100μg)、单纯CT(2μg),1次/wk,共4次,治疗结束后4wk处死动物,取胃黏膜行半定量细菌培养检查H.pylori情况.结果:治疗实验各组H.pylori根除率分别为:过氧化氢酶和黏附素保守区加CT组69.2%(18/26)、过氧化氢酶加CT组30.8%(8/26)、黏附素保守区加CT组38.5%(10/26);生理盐水组、单纯过氧化氢酶、单纯黏附素保守区、单纯CT组根除率均为0%.未根除H.pylori的小鼠,疫苗治疗组H.pylori的定植密度明显低于其他4组(P<0.05).此外,二价疫苗组的H.pylori根除率及定植密度较单价疫苗组均有显著性差异(P<0.05).结论:由过氧化氢酶和黏附素保守区加免疫佐剂组成的二价口服疫苗较单价口服疫苗有更好的免疫治疗效果,多价疫苗是未来H.pylori疫苗的研制方向.AIM： To investigate the effects of two valence vaccine consisting of Helicobacter pylori （ H. pylori） catalase and adhesin conservation in the treatment of H. pylori infection in an H. pylori infected mouse model. METHODS： C57BL/6 mice infected with H. pylori were divided into seven groups. The mice of each group were immunized respectively by catalase and adhesin conservation （separately, 100μg） plus CT （2μg）, catalase （100 μg） plus CT （2 μg）, adhesin conservation （100 μg） plus CT （2 μg ）, catalase （100 μg）, adhesin conservation （100 μg） and CT （2 μg）, PBS orally once a week for four weeks. Four weeks after the last treatment, all animals were sacrificed and stomach biopsies were collected to detect H. pylori by semi-quantitative bacterial culture assay. RESULTS： The eradication rate of two valence vaccine group, single va- lence vaccine group of catalase and single valence vaccine group of adhesin conservation was 69.2% （ 18/26）, 30.8% （8/26） and 38.5% （10/26）, respectively. The eradication rate of the other four groups was all 0%. The H. pylori colonized density of the vaccination group was significantly lower than that of the other four groups （ P 〈 0.05 ）. There were significantly differences in the eradication rate and H. pylori colonized density between the two valence vaccination group and the single valence vaccination groups （P 〈 0. 0.5）. CONCLUSION： The two valence vaccine consisting of catalase, adhesion conservation and adjuvant has better immunotherapeutic effects than the single valence vaccine. More H. pylori vaccine research should be directed to the multi-valence vaccine.

关 键 词：幽门螺杆菌 过氧化氢酶 黏附素 疫苗 

分 类 号：R377[医药卫生—病原生物学]