氦-氖激光诱变细菌细胞及原生质体选育果胶酶高产菌株  

The Breeding of High-Yield Pectinase Producing Bacteria Using He-Ne Laser Irradiation on the Cell and Protoplasts

作　　者：朱宏莉[1] 郭爱莲[1] 宋纪蓉[2] 杨秀芳[3] 张嘉[1] 张晓瑞[1] 

机构地区：[1]西北大学生命科学学院,西安710069 [2]西北大学化工学院,西安710069 [3]西安理工大学机械与精密仪器工程学院,西安710048

出　　处：《光子学报》2005年第11期1693-1696,共4页Acta Photonica Sinica

基　　金：陕西省教委基金资助项目(00JK138)

摘　　要：利用氦氖激光(波长632.8nm,功率15mW)诱变果胶酶产生菌的菌体细胞,获得了突变株ZHg,其酶活达到301u·mL-1,是出发菌株的1.3倍.进一步用氦氖激光照射ZHg的原生质体,从而得到了高产突变株ZHgA,其酶活达到357u·mL-1,比ZHg提高了18%,是出发菌株的1.6倍.该菌株在好氧和静置条件下均能良好产酶,经传代实验证明其产酶性能稳定.A mutant strain ZH-g was screened by He-Ne laser mutagenesis on the cells. Its pectinase activity was in 1.3 fold of the original one, reaching 301μ/mL. On the basis of this, using He-Ne laser irradiating on the protoplasts of ZH-g,the mutant strain ZH-gA with high yield of pectinase was obtained. It accumulated pectinase activity up to 357μ/mL which was 18% higher than that of the parent strain. This strain can produce pectinase efficiently under both aerobic and static cultivation. The enzyme activity is stable after several times of batch.

关 键 词：果胶酶 细菌 HE-NE激光 原生质体 诱变 

分 类 号：Q93[生物学—微生物学]