高效液相色谱法测定猪血浆中多西环素的含量  

Determination of doxycycline in pig plasma by HPLC

作　　者：蔡金华[1] 顾欣[1] 刘雅妮[1] 

机构地区：[1]上海市兽药饲料监察所,上海201103

出　　处：《中国兽药杂志》2005年第11期11-13,共3页Chinese Journal of Veterinary Drug

摘　　要：建立了高效液相色谱法测定猪血浆中多西环素含量的方法.采用Agillent 1100 HPLC系统,分析柱为ZORBAX SB C-18,流动相组分为超纯水(含0.6 mmoL/L EDTA·2Na,0.15%三氟乙酸,10 mmoL/L草酸)和乙腈,梯度洗脱,柱温15℃,检测波长350 nm.猪血浆样品经EDTA-McIlvaine缓冲液和20%(V/V)高氯酸的混合液缓中变性处理,取上清液直接进样测定.结果表明,血浆中多西环素的线性范围为0.10～12.80μg/mL,其方程为y=49.846 x-0.289,相关系数为0.999 2(n=8);空白血浆添加低、中、高浓度多西环素(0.10、0.80、6.40μg/mL)样品的平均回收率分别为94.0%、98.2%、100.5%(n=4);平均批内变异系数分别为2.8%、4.1%和1.2%(n=4),批间变异系数分别为6.5%、9.4%和3.4%(n=16).本方法样品前处理简单、快速,结果准确、可靠,适用于猪血浆中多西环素含量的测定及相关药代动力学研究.A simple and accurate HPLC method with DAD detector at 350 nm was developed for the determination of doxycycline in pig plasma. The sample preparation procedures included denaturalization of protein with the mixture of EDTA-McIlvaine buffer and 20% （V/V） perchloric acid, and precipitation of protein by freezing centrifuging. The standard curve function of doxycycline was y =49. 846x -0.289 in the range of 0.10 - 12.80 μg/mL with r = 0. 9992, n = 8. The precision of the method was determined. The mean recoveries of the plasma samples added doxycycline to 0.10,0.80 and 6.40 μg/mL were 94.0% ,98.2%, and 100.5% with coefficient of variation of 2.8 % ,4.1% and 1.2% （ n = 4 ） in one batch, and 6.5 %, 9.4% and 3.4 % （ n = 6 ） in different batches, respectively. This method offered a rapid, repeatable and accurate procedure and can be used to determine the concentration of doxycycline in pig plasma and the pharmacokinetic parameters of doxycycline in pig plasma.

关 键 词：高效液相色谱法 多西环素 猪 血浆 

分 类 号：S859.84[农业科学—临床兽医学]