柴芩平胃胶囊对反流性胃炎胃粘膜细胞凋亡及调控基因的影响  

Effect of Chai Qin Pingwei Capsule on Gastric Mucosal Cell Apoptosis and Regulatory Genes in Rats with Bile Reflux Gastritis

作　　者：杨鸿[1] 侯家玉[2] 田德禄[2] 

机构地区：[1]北京师范大学资源药物与中药资源研究所,北京100088 [2]北京中医药大学中药药理系,北京100029

出　　处：《广州中医药大学学报》2005年第6期462-465,共4页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：[目的]观察柴芩平胃胶囊(CQPC)对反流性胃炎模型大鼠胃粘膜细胞凋亡及调控基因的影响。[方法]Wistar大鼠随机分为假手术组,模型组,CQPC高、中、低剂量组(剂量分别为16.66、8.33、4.17g/kg),小柴胡冲剂组(10g/kg);除假手术组外,均采用B-Ⅱ式胃部分切除(胃空肠吻合)术复制大鼠反流性残胃炎模型,各组按设计剂量灌胃给药,连续4 周;分别采用ELISA、原位杂交、免疫组化方法观察CQPC对胃粘膜细胞凋亡及调控基因p53 mRNA、Bax和Bcl-2表达的影响。[结果]模型组结果显示胆汁反流可引起模型大鼠胃粘膜细胞凋亡增加,野生型p53 mRNA、Bax蛋白表达上调,Bcl-2 蛋白表达下调,与假手术组比较具有显著性差异(均P<0.05或P<0.01);高、中、低剂量CQPC均可减少胆汁反流引起的细胞凋亡,减少野生型P53 mRNA、Bax蛋白表达,增加Bcl-2蛋白表达(均P<0.05或P<0.01)。[结论]柴芩平胃胶囊治疗反流性胃炎的作用机制,可能与减少胃粘膜细胞凋亡,调节各种凋亡调控基因的表达有关。[Objective] To observe the effect of Chai Qin Pingwei Capsule （CQPC） on gastric mucosal cell apoptosis and regulatory genes in rats with bile reflux gastritis. [ Methods] Wistar rats were randomized into 6 groups： shamoperation group （A）, model group （B）, CQPC groups in the dosages of 16.66 （high）, 8.33 （moderate）, and 4.17 （low） g/kg respectively （C, D and E respectively）, Xiao Chaihu Granules group in the dosage of 10 g/kg （F）. Except the sham-operation group, the rats in other groups received B- Ⅱ gastrojejunostomy to induce bile reflux gastritis and were treated with gastric gavage of corresponding drugs according to the experimental design for 4 weeks. Effects of CQPC on gastric mucosal cell apoptosis and the expression of p53 mRNA, Bax and Bcl-2 were observed by enzyme-linked immunosorbent assay （ELISA）, hybridization in situ and immunohistochemistry method. [Results] Bile reflux in the model group caused the increase of gastric mucosal cell apoptosis, the up-regulation of wild-type p53 mRNA and Bax protein expression, and the down-regulation of Bcl-2 protein expression, the difference being significant as compared with the sham-operation group （P 〈 0.05 or P 〈 0.01）. CQPC in high-, moderate-and low-dosage had counteractive action against the above changes （P 〈 0.05 or P 〈 0.01 compared with the model group）. [ Conclusion ] The therapeutic effect of CQPC for bile reflux gastritis may be related to the decrease of gastric mucosal cell apoptosis and the regulation of apoptotic regulatory genes.

关 键 词：柴芩平胃胶囊/药理学 胃炎/中药疗法 细胞凋亡 基因表达调控 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]