应用聚合酶链反应技术检测广佛手黄龙病病原的研究  

Detection of Huanglongbing Pathogen in Citrus medica L. var. sarcodactylis (Noot.) Swingle by Polymerase Chain Reaction Analysis

作　　者：贺红[1] 潘超关 吴立蓉[1] 黄海波[1] 张桂芳[1] 

机构地区：[1]广州中医药大学中药学院,广州510405

出　　处：《广州中医药大学学报》2005年第6期479-481,共3页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(编号:020792)国家中医药管理局研究课题(编号:02-03ZP48)

摘　　要：[目的]建立鉴定广佛手黄龙病病原的有效方法,为无毒苗木的选育及病害防治提供早期诊断依据。[方法]在广佛手主要种植区进行调查,了解黄龙病的发病状况;根据柑桔黄龙病病原的DNA序列设计特异性的引物,对样品的DNA进行聚合酶链式反应(PCR)扩增。[结果]广东肇庆地区的广佛手出现类似柑桔黄龙病的症状,PCR检测结果表明,在表现发病症状的广佛手样品中有403 bp的特异性DNA片段出现,而健康的样品中则没有扩增出特征带,表明广佛手的感病症状是由黄龙病病原引起的。[结论]PCR技术是广佛手黄龙病病原鉴定的有效方法之一。[Objective] To establish an effective way for the detection of Huanglongbing pathogen in Citrus medica L. var. sarcodactylis （Noot.） Swingle by polymerase chain reaction （PCR） analysis, and to provide evidence for the early diagnosis of the culture of pathogen-free plantlets and for the prevention and control of diseases. [ Methods ] The incidence of Huanglongbing disease was investigated in the main producing area of Citrus medica L. var. sarcodactylis （Noot.） Swingle. By using the special primers designed by the DNA sequence of Citrus Huanglongbing pathogen, PCR amplification of DNA from the samples was conducted. [ Results ] Symptoms similar to the Citrus Huanglongbing disease occurred in Citrus medica L. var. sarcodactylis （Noot.） Swingle growing in Zhaoqing, Guangdong. The results of PCR analysis showed that a special DNA fragment of 400bp had been detected in the affected plants of Citrus medica L. var. sarcodactylis （Noot.） Swingle, but it was not found in the healthy plants, indicating that the symptoms were caused by the Huanglongbing pathogen. [ Conclusion ] PCR analysis is an effective way for the detection of Huanglongbing pathogen in Citrus medica L. var. sarcodactylis （Noot.） Swingle.

关 键 词：广佛手/微生物学 黄龙病/诊断 生物学鉴定法 

分 类 号：R282.2[医药卫生—中药学]