理气化痰祛瘀法对非酒精性脂肪肝大鼠血清FFA TNF-α IL-6的影响  

Effects of Regulating Qi to Dissipate Blood Stasis and Phlegm on Serum FFA-TNF-α and IL-6 in Ras with Nonalcoholic Fatty Liver

作　　者：严茂祥 陈芝芸 孙丽伟 何蓓晖 项柏康 

机构地区：[1]浙江中医学院附属医院,浙江杭州310006

出　　处：《中医药学刊》2005年第11期1979-1981,共3页Study Journal of Traditional Chinese Medicine

基　　金：浙江省教育厅资助项目(20020080)

摘　　要：目的观察理气化痰祛瘀中药对高脂饮食诱导的脂肪肝大鼠血清游离脂肪酸(FFA)、肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)的影响,探讨其可能作用机理。方法以高脂饮食喂养Wistar大鼠12周,建立大鼠非酒精性脂肪肝模型,以不同剂量的理气化痰祛瘀中药治疗8周,常规HE染色观察肝组织的病理改变,比色法检测血清FFA含量,放免法测定血清TNF-α、IL-6的含量。结果模型组大鼠血清FFA、TNF-α、IL-6含量较正常组明显升高。理气化痰祛瘀法中药能明显改善肝细胞的脂肪变性情况(P<0.05),显著降低血清中FFA、TNF-α、IL-6含量(P<0.01,P<0.05)。结论FFA、TNF-α、IL-6参与高脂饮食诱导的大鼠非酒精性脂肪肝的发生,理气化痰祛瘀法中药能显著降低大鼠血清FFA、TNF-α、IL-6水平,防止脂肪肝进一步发展。Objective：To investigate the effects of regulating qi to dissipate blood stasis and phlegm on serum FFA, TNF - α and IL - 6 in rats with nonalcoholic fatty liver. Methods ： The model of nonalcoholic fatty liver in Wistar rats were induced by a term of 12 - week high fat diet, and were treated by different doses of Chinese Herbs with regulating qi to dissipate blood stasis and phlegm after a treatment of 8 weeks, the pathological changes were observed with HE staining, the amount of serum FFA was measured with colorimetric method and the amounts of serum TNF - α and IL - 6 were detected with RIA. Results ： Compared with the normal group, the levels of serum FFA, TNF - α and IL - 6 of rats in the model group were obviously increased, the Chinese Herbs could improve the steatosis of hepatocyte（ P 〈 0.05 ）, and remarkably decrease the amounts of serum TNF - α and IL - 6. Conclusion ： FFA, TNF - α and IL - 6 be concerned with the pathogenesis of experimental nonalcoholic fatty liver induced by high fat diet, the Chinese Herbs can lower the levels of FFA, TNF - α and IL - 6, prevent the further development of fatty liver.

关 键 词：脂肪肝 细胞因子 理气化痰祛瘀法 大鼠 

分 类 号：R259.755[医药卫生—中西医结合]