用原位核酸杂交法探讨柯萨奇B组病毒与云南慢型克山病的关系  

Relationship between Coxsackie Virus B and chronic Keshan disease in Yun nan Province by using hyhridization in situ

作　　者：牛美娟[1] 钟学宽[2] 郭彩玲[1] 邹宁[2] 刘继海 曲章义[1] 张凤民[1] 周令望[2] 王福文 牛存龙 张德全[2] 李绍贤[1] 谷鸿喜[1] 

机构地区：[1]哈尔滨医科大学微生物教研室,150086 [2]哈尔滨医科大学克山病研究所 [3]云南省克山病防治研究中心

出　　处：《中国地方病学杂志》1996年第2期65-67,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助课题 批号 3937065

摘　　要：为了探讨柯萨奇 B 组病毒感染与云南慢型克山病的关系,本文用缺口平移法制备生物素标记的柯萨奇 B_3病毒的 cDNA 探针与云南慢型克山病尸检心肌组织15例及脑外伤等非正常死亡的尸检心肌组织10例石腊切片及引产胎儿心肌组织10例的石腊切片进行原位杂交。结果云南慢克16例中有11例出现阳性杂交信号,即有柯萨奇 B 组病毒(CVB)RNA 存在,阳性率达73.3%。脑外伤等非正常死亡的心肌及胎心均为阴性。提示云南慢型克山病与 CVB 的感染高度相关。We performed hybridization in situ to examine RNA of Coxsackie Virus B(CVB)in paraffin-em- bedded myocardial specimens from 15 patients with chronic Keshan disease in Yunnan Province,10 pa- tients with cerebral trauma and 10 fetuses from non-endemic areas using the CVB_3c DNA probe la- belled with Bio-11-dUTP by nick translation.OF the 15 patients with chronic Keshan disase,11(73. 3%)showed positive findings.Their myocardial tissues were infected by CVB previously.The results suggested that CVB infeetion may be highly related with chronic Keshan disease in Yunnan province

关 键 词：柯萨奇B组病毒 克山病 原位核酸杂交 

分 类 号：R542.302[医药卫生—心血管疾病]