用原位核酸杂交法探讨柯萨奇B组病毒与黑龙江慢型克山病的关系  

Relationship between Coxsackie Virus B and chronic Keshan disease in Heilongjiang province by using hybridization in situ

作　　者：周令望[1] 钟学宽[1] 牛美娟[2] 郭彩玲[2] 邹宁[1] 曲章义[2] 崔玉娥[2] 康向华[3] 刘阳[1] 刘艺[1] 杨晓霞[4] 付桂玲[1] 

机构地区：[1]哈尔滨医科大学克山病研究所,150086 [2]哈尔滨医科大学微生物教研室 [3]哈尔滨医科大学法医教研室 [4]山东省地方病防治研究所

出　　处：《中国地方病学杂志》1996年第2期68-70,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金课题 批准号 39370615

摘　　要：克山病是原因未明的地方性心肌病,慢型克山病有关病毒病因的研究未见有关报道,作者用生物素标记的柯萨奇 B_3病毒的 cDNA 探针与黑龙江病区慢型克山病尸检心肌组织及非正常死亡成人心肌组织和非病区7～8个月引产胎儿心肌组织进行了原位杂交,以检测柯萨奇 B 组病毒 RNA。结果13例慢型克山病中有9例呈现阳性杂交信号,阳性率达69.2%,两对照组均为阴性。表明黑龙江慢型克山病心肌组织中有柯萨奇 B 组病毒 RNA 的存在,提示黑龙江病区慢型克山病与柯萨奇 B 组病毒的感染呈现高度相关。The Keshan disease is an endemic myocardal disease and its pathogenesis is still unknown.The re- porto about the viral cause of keshan disease were not seen.We performed hybridization in situ to exam- ine RNA of coxsackie Virus B(CVB)in paraffin-embedded myocardial specimens from 13 patients with chronic Keshan discase,10 patients with cerebral trauma or carbon monoxide poisoning and 10 foetuses from non-end emic areas using the CVB_3 cDNA probe labelled with Bio-11-dUTP by nick transla- tion.OF the 13 patients with chronic Keshan disease,9(69%)showed positive findings.It was suggested that there might be CVB RNA in myocardial tissues of chronic Keshan disease in Heilongjiang province, and the development of chronic Keshan disease in Heilongjiang may be highly related with persistent in- fection of CVB.

关 键 词：柯萨奇B组病毒 原位核酸杂交 克山病 

分 类 号：R542.302[医药卫生—心血管疾病]