皮下盘菌属及其近似类群RAPD-PCR反应条件的优化  

Optimization of RAPD-PCR Reaction Conditions for Hypoderma and Its Similar Groups

作　　者：何宇峰[1] 王士娟[2] 叶光斌[2] 林英任[1] 

机构地区：[1]安徽农业大学林学与园林学院,安徽合肥230036 [2]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《菌物研究》2005年第2期1-5,共5页Journal of Fungal Research

基　　金：国家自然基金资助项目(30370011;3470014)

摘　　要：以悬钩子皮下盘菌等总基因组DNA为皮下盘菌属(Hypodermade Not.)及其近似类群RAPD体系优化的模板,对模板DNA浓度、dNTPs浓度、Mg2+浓度、引物浓度、TaqDNA聚合酶浓度等反应体系以及退火温度和时间、延伸时间、循环次数等扩增程序条件进行优化,寻找出适合此类菌物RAPD-PCR反应的最佳反应体系和最佳扩增程序。Genomic DNA of Hypoderma rubi was regarded as template optimized RAPD system of Hypoderma and its similar groups. The influencing factors and experimental parameters on RAPD were studied, including templates, dNTPs, Mg^2 ＋ , primers, Taq DNA polymerase, annealing temperature and time, elongation time and cycle numbers. Optimal reaction system and amplification procedure were selected to apply to RAPD-PCR reaction of Hypodenna and its similar groups.

关 键 词：皮下盘菌属 齿裂菌属 散斑壳属 RAPD-PCR反应条件 优化 

分 类 号：Q939.5[生物学—微生物学]