散斑壳属部分种内及近似种间的RAPD分析  

RAPD Analysis of Some Intraspecific and Similar Interspecific Strains of Lophodermium

作　　者：叶光斌[1] 余盛明 何宇峰[3] 王士娟[1] 林英任[3] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽省黄山风景区管委会,安徽黄山242709 [3]安徽农业大学林学与园林学院,安徽合肥230036

出　　处：《菌物研究》2005年第2期27-32,共6页Journal of Fungal Research

基　　金：国家自然科学基金项目(30370011;30470014)

摘　　要：从100个随机引物中选取重复性好、条带清晰的随机引物分别对针叶树和阔叶树上的34个散斑壳(Lophodermiumspp.)菌株进行RAPD-PCR扩增反应,RAPD分析结果与表型性状表现出良好的一致性,从而表明RAPD技术可以较好的应用于散斑壳属种内及近似种间亲缘关系的分析。通过RAPD分析结果与表型性状的比较和分析,明确了部分表型性状在散斑壳属近似种区分方面的重要性。Thirty-four Lophodermium strains on conifers and broadleaf trees were amplified by RAPD- PCR reaction. The primers used in the reaction were selected from 100 random primers and their gel eleetrophoresis diagram can be amplified repeatedly and clearly. The results of RAPD analysis cohered with its phenotypic characters quite well, which indicated that RAPD technology could analyze kinships between intraspecies and similar interspecies of Lophodermium efficiently. By the comparison of RAPD analytic results and its phenotypic characters, the importance of partial phenotypic characters were affirmed in discrimination of similar species of Lophodermium.

关 键 词：散斑壳属 RAPD分析 亲缘关系 

分 类 号：Q939.5[生物学—微生物学]