姜黄素诱导人肺腺癌A549细胞脱落凋亡作用的研究  

Curcumin induced anoikis of lung cancer cells A549

作　　者：张艰[1] 李圣青[1] 吴昌归[1] 李志奎[1] 柏长青[1] 戚好文[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710032

出　　处：《中国药理学通报》2005年第11期1332-1335,共4页Chinese Pharmacological Bulletin

基　　金：军队青年基金资助项目(No01Q125);陕西省自然科学基金资助项目(No2003C2004)

摘　　要：目的检测姜黄素诱导人肺腺癌细胞(A549)脱落凋亡的作用。方法采用DNA ladder法,流式细胞仪(FCM)技术结合PI及AnnexinV-FITC双标记染色试验方法及W estern-b lot法观察姜黄素是否能诱导A549脱落凋亡。结果A549细胞具有抗脱落凋亡的特性,而10μmol.L-1的姜黄素即可引起悬浮培养的A549细胞发生脱落凋亡。结论姜黄素可诱导人肺腺癌细胞(A549)脱落凋亡。Aim To study the induetive effect of eureumin on the anoikis of lung cancer cells A549.Method DNA laddering assay,FCM combined with PI and AnnexinV-FITC double pigmention and Western-blot method were used to observe the inductive effect of curcumin on anoikis. Result A549 cells had the pecuiarity of anoikis resistance, and curcumin at concentration of 10μmol·L^-1 remarkably induced apoptosis of suspended A549 cancer cells. Conclusion Curcurain can induced anoikis of the lung cancer cell A549.

关 键 词：姜黄素 A549人肺腺癌细胞 脱落凋亡 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]