阿托伐他汀对兔动脉粥样硬化中NF-κB/IκB和炎症因子表达的影响  

Effect of atorvastatin on NF-κB/IκB signal pathway and inflammation factor expression in rabbit atherosclerosis

作　　者：杜林林[1] 王朝晖[2] 

机构地区：[1]温州医学院附属第一医院急诊科,浙江温州325000 [2]华中科技大学同济医学院附属协和医院心内科,湖北武汉430022

出　　处：《中国药理学通报》2005年第11期1392-1395,共4页Chinese Pharmacological Bulletin

摘　　要：目的探讨高脂饮食诱发兔动脉粥样硬化中核因子-κB(NF-κB)的活化与其抑制因子IκB的表达,以及阿托伐他汀对NF-κB/IκB信号途径、ICAM-1和P选择素的影响。方法24只新西兰♂家兔随机分为正常对照组、高胆固醇组和阿托伐他汀组,应用W estern b lot方法检测动脉中胞核NF-κB p65亚基和胞浆IκBα表达变化;免疫放射法检测血清P选择素水平,免疫组织化学检测血管壁ICAM-1的表达。结果高胆固醇组与对照组比较胞核NF-κB p65和血管壁ICAM-1表达明显增强、血P选择素明显升高(P<0.05),胞浆中IκBα表达明显减弱(P<0.05);阿托伐他汀组与高胆固醇组比较NF-κB p65和血管壁ICAM-1明显表达较弱、血P选择素明显减少(P<0.05),胞浆IκBα的表达增强(P<0.05)。结论NF-κB/IκB信号途径在动脉粥样硬化中起重要作用,阿托伐他汀可以减少P选择素、ICAM-1的表达。Aim To explore the effect of atorvastatin on NF-KB/IKB signal pathway, P selectin and ICAM-1 expression in cholesterol-rich diet-induced rabbit atherosclerosis. Methods 24 New Zealand white rabbits were randomly divided into three groups： control group, high-cholesterol group and atorvastatin group. Nucleus NF-KB p65 and cytoplasma IκBα were detected with Western blot technique. Plasma P selectin was detected with Immunoradioassay. Immunohistochemistry was employed to detect the expression of ICAM-1. Results Compared with the control group ,the expression of nucleus NF-KB p65, P selectin and ICAM-1 increased significantly and cytoplasma IκBα reduced in high-cholesterol group （ P 〈 0. 05 ）. Atorvastatin reduced the expression of nucleus NF-κB p65, P selectin and ICAM-1 and increased the expression of cytoplasm IκBα, as compared with high-cholesterol group （P 〈 0.05 ）. Conclusions NF-κB/IκB signal pathway plays a role in atherosclerosis. Atorvastatin can reduce the expression of p selectin and ICAM-1 in atherosclerosis.

关 键 词：阿托伐他汀 核因子ΚB 动脉粥样硬化 实验药理学 

分 类 号：R972.6[医药卫生—药品]