儿宝颗粒对厌食大鼠下丘脑外侧区神经元整合摄食相关信息的影响  

Effect of Er’bao Granule on Integration of Ingestion Behavior-related Information by Neurons in Lateral Hypothalamic Area of Anorexia Rats

作　　者：张月萍[1] 杜永平[1] 张国成[1] 

机构地区：[1]第四军医大学西京医院,西安710032

出　　处：《中国中西医结合杂志》2005年第11期996-999,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.39670896和30271634)

摘　　要：目的证实儿宝颗粒影响下丘脑外侧区神经元对外周传入信号的敏感性,阐明儿宝颗粒促进摄食行为的中枢机制。方法用特制饲料喂养大鼠1周制备厌食模型,用儿宝颗粒灌胃治疗3周,然后用电生理技术细胞外记录法,记录大鼠下丘脑外侧区神经元的自发放电,观察其对电刺激胃迷走神经和静脉注射葡萄糖注射液的反应,比较正常组、模型组和治疗组3组间的异同。结果造模7天时模型组和治疗组大鼠进食量低于正常组(P<0.01),造模21、28天时治疗组大鼠进食量与正常组比较差异无显著性。与正常组比较,模型组那些接受胃迷走神经传入的下丘脑外侧区神经元中,血糖敏感神经元所占比例显著降低(P<0.01);而治疗组该指标与正常组比较差异无显著性。结论儿宝颗粒能够调节摄食中枢神经元对外周传入信号的敏感性,从而影响摄食中枢神经元对多种传入信息的整合。Objective To confirm the effect of Er＇bao granule （EBG） on the sensitivity to peripheral afferent signal of neurons in lateral hypothalamic area （LHA） to illustrate the central mechanism of EBG in promoting ingestion behavior. Methods The anorexia rat model was established by feeding special prepared forage for one week, and all the model rats were administrated with EBG by gavage for 3 weeks. The spontaneous discharge of LHA neurons was recorded using electro-physiological extracellular recording method, and its response to electrical stimulus on gastric vagus nerve and intravenous injection of glucose were observed and compared a- mong the normal, model and treated groups. Results As compared with the normal group, among the LHA neurons responding to afferent gastric vagal impulse, the proportion of glycemiasensitive neurons in the model group was significantly decreased （P 〈 0.01）, but insignificant difference was shown in comparison between the treated group and the normal group. Conclusion EBG play a role in regulating the sensitivity of LHA neurons to peripheral afferent signal and thus to influence the multi-afferent information integration of ingestion central neurons.

关 键 词：儿宝颗粒 厌食 下丘脑外侧区 血糖敏感神经元 胃迷走神经 

分 类 号：R285[医药卫生—中药学]