益气活血法对多发脑梗死性痴呆模型大鼠脑神经细胞凋亡调控基因的影响  

Study on Effect of Benefiting Vital Qi and Promoting Blood Circulation in Affecting Gene Expression Related to Appotosis of Neuron in Rat Model of Multiple Infarctional Dementia

作　　者：周建英[1] 许冬青[1] 王明艳[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《中国中医药信息杂志》2005年第11期38-39,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：江苏省教育厅自然科学基金资助项目(99KJB360006)

摘　　要：目的观察益气活血法对多发脑梗死性痴呆模型大鼠脑神经细胞凋亡调控基因的影响。方法采用颈内动脉注射血栓的方法,复制多发脑梗死性痴呆大鼠模型,观察益气复智颗粒24.84、12.4、6.21g/kg灌胃对实验动物脑组织bax和bcl-2基因蛋白表达及bax/bcl-2的影响。结果益气复智颗粒能抑制脑缺血后脑组织中bax基因蛋白的过度表达,并明显降低bax/bcl-2的比值。结论益气活血法及益气复智颗粒具有较好的调控脑缺血后基因蛋白表达的能力,可能是其抑制脑神经元凋亡的主要机理之一。Objective To observe the effect of Benefiting vital Qi and Promoting Blood Circulation in affecting gene expression related to appotosis of neuron in rat models with multiple infarctional dementia. Methods Internal carotid injection method was used to make rat models with multiple infarctional dementia and observations were made to observe effects in affecting expression of gene bax, gene bcl-2 and the ratio of bax to bcl-2 in the brain tissue of rat by small, moderate and large concentration of Yiqi Fuzhi Granule. Results Yiqi Fuzhi Granule could inhibit the overexpression of bax gene in brain tissue after brain ischemia. Conclusion Benefiting vital Qi and Promoting Blood Circulation and Yiqi Fuzhi Granule could regulate expression of gene after brain ischemia. This may be the main mechanism of resisting the appotosis of neuron.

关 键 词：多发脑梗死性痴呆 神经细胞凋亡 bax bcl-2 BAX/BCL-2 益气活血法 

分 类 号：R285.5[医药卫生—中药学]