Smad4基因敲除对小鼠听力和前庭功能的影响  

Effect of Smad4 Knockout on Hearing and Vestibular Function of Mouse

作　　者：卢云云[1] 杨仕明[1] 郭维维[1] 胡吟燕[1] 杨伟炎[1] 杨晓[2] 

机构地区：[1]解放军总医院耳鼻咽喉-头颈外科,解放军耳鼻咽喉科研究所,北京100853 [2]军事医学科学院生物工程研究所发育和疾病遗传学研究室

出　　处：《听力学及言语疾病杂志》2005年第6期424-426,共3页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金(30000189);军队十五科研基金(01Q050);北京市自然科学基金(7042061)资助

摘　　要：目的研究Smad4基因敲除(Smad4+/-)小鼠听力和前庭功能的改变。方法检测Smad4基因敲除小鼠(1、2、3、6月龄)与同种同月龄的野生型(Smad4+/+)小鼠的ABR阈值。按Steal的方法测定小鼠的平衡功能,包括游泳试验、空间姿势反射,以及一般行为观察。结果2、3、6月龄Smad4+/-小鼠听力较同种野生型小鼠明显下降,两种基因型小鼠听阈有显著性差异(P<0.01),但1月龄的不同基因型小鼠听力水平无统计学差异(P>0.05)。Smad4基因敲除小鼠与野生型小鼠外观和体重无明显差异(P>0.05),生后至6月龄的Smad4+/-小鼠与野生型小鼠均无前庭功能异常和行为异常。结论单倍体Smad4基因敲除小鼠听力明显障碍,但前庭功能基本正常。表明Smad4对内耳的听觉有重要作用,可能存在着单倍体功能不全性;而单倍体Smad4基因缺失对于前庭功能影响可能不明显。Objective To study the effect of Smad4 knockout on functions of mouse inner ear. Methods ABR threshold was measured in Smad4 knockout mouse aged 1,2,3 and 6 months and wild - type mouse of same age. The methods of Steal was used to measure the balance functions of mouse （including swimming test and space posture reflex） and some general behaviors of mouse were observed. Results In the mouse group aged 2,3 and 6 months, the hearing of Smad4 ＋ / - mouse was evidently poorer than that of wild- type mouse of same kind, and significant difference there was in the auditory threshold of both groups mouse（ P 〈 0.01 ）. As for Smad4 （ ＋ / - ） mouse and wild - type mouse aged from birth to 6 months, there was not abnormal in general behaviors, swimming test and space pasture reflex examination did not appear abnormal. Conclusion The hearing of Smad4 knockout mouse decreases severely but its vestibular function is normal, which indicates that Smad4 gene plays an important role in the heating function of inner ear, but the deficiency in Smad4 gene maybe have no evident influence on vestibular function.

关 键 词：SMAD4 基因敲除 内耳 听功能 前庭功能 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]