Immune Responses to Six Synthetic Peptides of Capsid Protein with Sera from HIV-1 Infected Individuals  

作　　者：Guangjie Liu Liumeng Yang Jianhua Wang Gaohong Zhang Xiangmei Chen Yongtang Zheng 

机构地区：[1]Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China [2]The Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Cellular & Molecular Immunology》2005年第4期289-293,共5页中国免疫学杂志（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(39500137);the Natural Science Foundation of Yunnan(95C0099Q);Key Scientific and Technological projects of China(2004BA719A14)and Yunnan(2004NG12);CAS Projects(STZ-01-17,KSCX2-SW-216,KSCXl-SW-1l);National 863 Program(2003AA2 19142).

摘　　要：Many B cell epitopes within p24 of human immunodeficiency virus type 1 （HIV-1） were identified, while most of them were determined by using murine monoclonal antibodies reacting with overlapping peptides of p24. Therefore these epitopes may not represent the actual epitopes recognized by the HIV-1 infected individuals. In the present study, immune responses of 67 HIV-1 positive sera from Yunnan Province, China to five peptides on p24 of HIV-1 and one of HIV-2 were analyzed. All of 67 sera did not recognize peptide GA-12 on HIV-1 and peptide AG-23 on HIV-2, which indicated that GA-12 was not human B cell epitope and AG-23 did not cross-react with HIV-1 positive serum. Except 13 sera （19.4%）, all remaining sera did not recognize peptides NI-15, DR-16, DC-22 and PS-18, which indicated that these four peptides represented B cell linear epitopes of HIV-1 p24 in some HIV-1 infected individuals but not the immuno-dominant epitopes in most individuals.Many B cell epitopes within p24 of human immunodeficiency virus type 1 （HIV-1） were identified, while most of them were determined by using murine monoclonal antibodies reacting with overlapping peptides of p24. Therefore these epitopes may not represent the actual epitopes recognized by the HIV-1 infected individuals. In the present study, immune responses of 67 HIV-1 positive sera from Yunnan Province, China to five peptides on p24 of HIV-1 and one of HIV-2 were analyzed. All of 67 sera did not recognize peptide GA-12 on HIV-1 and peptide AG-23 on HIV-2, which indicated that GA-12 was not human B cell epitope and AG-23 did not cross-react with HIV-1 positive serum. Except 13 sera （19.4%）, all remaining sera did not recognize peptides NI-15, DR-16, DC-22 and PS-18, which indicated that these four peptides represented B cell linear epitopes of HIV-1 p24 in some HIV-1 infected individuals but not the immuno-dominant epitopes in most individuals.

关 键 词：HIV-1 human serum immune response P24 synthetic peptide 

分 类 号：R512.91[医药卫生—内科学]