低密度脂蛋白免疫复合物对单核细胞源性巨噬细胞胆固醇代谢及低密度脂蛋白受体表达的影响  

Effects of LDL-Containing Immune Complexes on Cholesterol Metabolism and LDL Receptor Expression in Monocyte-Derived Macrophages

作　　者：倪晓晴[1] 朱健华[2] 孙承龙[3] 

机构地区：[1]南通大学附属医院老年医学科 [2]南通大学附属医院心内科 [3]南通大学附属医院医学检验中心,江苏省南通市226001

出　　处：《中国动脉硬化杂志》2005年第4期461-463,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的研究动脉粥样硬化过程中低密度脂蛋白免疫复合物对单核细胞源性巨噬细胞胆固醇代谢和低密度脂蛋白受体表达的影响。方法以佛波酯刺激分化的THP1细胞作为单核细胞源性巨噬细胞模型。通过胆固醇酶联法测定细胞内胆固醇含量,逆转录—聚合酶链反应检测细胞低密度脂蛋白受体mRNA的表达,Westernblot检测细胞低密度脂蛋白受体蛋白的表达。结果与低密度脂蛋白组、阴性组及IgG免疫复合物组比较,低密度脂蛋白免疫复合物组细胞内胆固醇水平明显增高,并且低密度脂蛋白受体mRNA及蛋白的表达增加。结论低密度脂蛋白免疫复合物能显著增加巨噬细胞内胆固醇含量,亦能够促进低密度脂蛋白受体mRNA与蛋白的表达,提示低密度脂蛋白免疫复合物在动脉粥样硬化进展过程中起重要的作用。Aim To study the effects of low density lipoprotein-immune complexes （LDL-IC） on the cholesterol metabolism and on the expression of low density lipoprotein receptor （LDLR） in monoeyte- derived macrophanges. Methods PMA-treated THP-1 cell were used as a model of monoeyte-derived macrophages. Quantitative analysis of intracellular cholesterol content was performed by cholesterol enzyme link assays. The expression of LDLR mRNA was detected by reverse transcription polymerase chain reaction（RT-PCR） and LDLR protein was detected by Western blot. Results The insular cholesterol level treated with LDL-IC was higher than other groups treated with LDL, IgG-IC and negative control. Both LDLR mRNA and LDLR protein values of macrophages stimulated by LDL-IC were significantly enhanced. Conclusions Incubating with LDL-IC can not only markedly elevate the intracellular cholesterol content but also promote the expression of LDLR mRNA and protein which suggest LDL-IC may be an important factor during the development of atherosclerosis.

关 键 词：生物化学 脂蛋白免疫复合物致动脉硬化作用 低密度脂蛋白 免疫复合物 单核细胞源性巨噬细胞 低密度脂蛋白受体 动脉粥样硬化 

分 类 号：Q5[生物学—生物化学]