载脂蛋白C3基因多态性-482C>T与血浆脂质的关系  

Relationship Between the Polymorphism of Apolipoprotein C3 Gene -482C>T and the Levels of Serum Lipids and Lipoproteins

作　　者：李国平[1] 陈保生[1] 薛红[1] 曾武威[1] 吴刚[1] 

机构地区：[1]中国医学科学院基础医学研究所,中国协和医科大学基础医学院生物化学系医学分子生物学国家重点实验室脂蛋白组,北京市100005

出　　处：《中国动脉硬化杂志》2005年第3期335-339,共5页Chinese Journal of Arteriosclerosis

基　　金：国家重大基础研究项目973项目(G2000056902)资助

摘　　要：目的研究载脂蛋白C3基因上游调控区内-482C>T多态性位点与血浆脂质以及载脂蛋白水平的关系。方法应用聚合酶链反应—限制片长多态性方法逐个鉴定每个个体的基因型,并测定其血浆脂质和载脂蛋白水平。结果-482T等位基因携带者具有较高的甘油三酯水平(P=0.044);同时该等位基因的携带者也具有较高的载脂蛋白C2水平(P=0.017)。结论载脂蛋白C3基因-482C>T位点和血浆甘油三酯以及其它几种脂蛋白密切相关。Aim To investigate the relationship between the polymorphism of apolipoprotein C3 gene-482C 〉 T and the levels of serum lipids as well as lipoproteins. Methods The genotypes of the subjects were determined by using polymerase chain reaction-restriction fragments length polymorphisms （PCR-RFLP）, and the serum lipids and apolipoproteins were determined. Results The carrier with minor allele of -482C 〉 T have higher levels of serum triglycefides. At the same time the cartier with minor allele of- 482C 〉 T have higher levels of serum apolipoprotein C3 and higher apolipoprotein C2. Conelusions The results strongly support that the single nucleotide polymorphisms, - 482C 〉 T in apolipoprotein C3 gene, is related to the levels of serum triglyceride （TG） and high density lipoprotein cholesterol （HDLC） and those of other several lipoproteins in the Chinese population.

关 键 词：分子生物学 载脂蛋白C3基因多态性研究 聚合酶链反应-限制片长多态性 载脂蛋白C3 基因多态性 甘油三酯 

分 类 号：Q7[生物学—分子生物学]