戊二醛、钙离子对红细胞与内皮细胞粘附的影响  

Influence of Glutaraldehyde, Ca^(2+)on the Adhesion of RBCs to HUVEC

作　　者：黄勋[1] 王玲[1] 丁肇华 陈槐卿[1] 

机构地区：[1]华西医大基础医学院分析测试中心,华西医大生物医学工程研究室

出　　处：《生物医学工程学杂志》1996年第1期58-61,共4页Journal of Biomedical Engineering

摘　　要：采用流室系统研究成二醛、钙离子对红细胞与内皮细胞粘附的影响。流室中的流动是充分发展的一二维层流，能产生稳定的剪切应力。对粘附在内皮细胞上的红细胞（分别用１０、９μｍｏｌ／ｍｌ的钙预处理过的红细胞，分别用０．２％、０．８％的戊二醛预处理过的红细胞）依次施加＝０．１、０．２、０．４、０．５、１．０、１．５、２．０Ｐａ的切应力作用２分钟，再分别对粘附的红细胞计数。实验表明（１）切应力越大，粘附的红细胞越少；（２）戊二醛使红细胞变硬，红细胞粘附数减少；（３）低剂量Ｃａ（２＋）使红细胞粘附增多，高剂量Ｃａ（２＋）反而对红细胞粘附无显著影响。初步提示血液流变学因素（血流切应力）和红细胞的流变特性对红细胞的粘附有极大的影响。A flow chamber system, in which the flow is two-dimensional and fully developed laminar,was adopted in this study. We investigated the effect of glutaraldeyde, Ca2+ the adhesion of RBCs to HUVEC monolayer at shear stress levels 0. 1, 0. 2, 0. 3. 0. 4,0. 5, 1. 0, 1. 5, 2. 0 Pa respectively for a period of 2 minutes. By realtime observation and recordation system conjoined to phase contrast microscope,we counted the number of adherent RBCs at those levels of shear stress. The results were: (1)RBCs pretreated with glutaraldehyde adhered to HUVEC less and washed out easier than that of control group did; (2)low dose of Ca2+ increased the adhesion of RBCs, but high dose of Ca2+ did not affect the adherence of RBCs.

关 键 词：血液流变学 戊二醛 钙离子 红细胞 内皮细胞 

分 类 号：R446.11[医药卫生—诊断学]