妇科白带片中橙皮苷的含量测定  

Determination of Hesperidin in Fuke Baidai Tablets

作　　者：苏健俊[1] 丁野[1] 

机构地区：[1]湖南省药品检验所,湖南长沙410001

出　　处：《中国药业》2005年第11期34-35,共2页China Pharmaceuticals

摘　　要：目的:建立妇科白带片中橙皮苷的含量测定方法。方法:采用高效液相色谱法(HPLC法),C18柱,以甲醇-醋酸-水(35∶4∶61)为流动相,检测波长为283nm。结果:橙皮苷进样量在0.0396～0.3564μg范围内与峰面积线性关系良好,回归方程为A=2404.897C-0.0598,r=0.9999,平均加样回收率为97.67%。结论:HPLC法简便易行,且具有较好的选择性和准确性,可用于妇科白带片的质量控制。Objective： To establish a method of determination of hesperidin in Fuke Baidai tablets. Methods： In HPLC system, C18 column was used as stationary phase, the mobile phase was methol-acetic acid-water（35：4：61）, the detection wavelength was 283 nm. Results： The calibration curve of hesperidin was linear in the range of 0.0396 - 0.3564μg, A=2404.897C - 0.0598, r=0.9999. The average recovery was 97.67%. Conclusion： The method was simple, accurate and may be adopted for the quality control of Fuke Baidai tablets.

关 键 词：妇科白带片 橙皮苷 高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学]