HPLC-ELSD法测定黄芪生脉饮中黄芪甲苷含量  

Determination of Astragaloside Ⅳ in Huangqi Shengmai Yin by HPLC-ELSD

作　　者：裘飞君 王岳钧 俞建平[2] 

机构地区：[1]浙江新光药业有限公司,浙江嵊州312400 [2]浙江省药品检验所,浙江杭州310004

出　　处：《中国药业》2005年第11期37-38,共2页China Pharmaceuticals

摘　　要：目的:建立黄芪生脉饮的定量质控方法。方法:采用HPLC-ELSD法测定主药黄芪中黄芪甲苷的含量。结果:黄芪甲苷进样量在0.495～7.425μg范围内与峰面积呈良好的线性关系,平均回收率为97.9%,RSD=1.7%。结论:该方法简单,重现性好,可用于控制黄芪生脉饮的质量。Objective： To establish a method of determination of Huangqi Shengmai Yin. Methods. The content of astragaloside Ⅳ was determined by HPLC - ELSD. Results： A good linearity was observed for astragaloside Ⅳ in the range of 0.495 - 7.425μg. The average recovery of astragaloside Ⅳ was 97.9% with RSD=1.7%. Conclusion： HPLC-ELSD was simple and accurate with a good repeatability. It can be used for the quality control of Huangqi Shengmai Yin.

关 键 词：HPLC—ELSD 黄芪生脉饮 黄苠甲苷 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286[医药卫生—中医学]