腺病毒介导hIL-2基因转染膀胱上皮的体内研究  

Adenovirus mediated hIL-2 gene transfer to bladder epithelium in vivo

作　　者：顾正勤[1] 许传亮[1] 高旭[1] 孙颖浩[1] 李云[1] 沈茜[2] 

机构地区：[1]第二军医大学附属长海医院泌尿外科,上海200433 [2]第二军医大学附属长海医院检验科

出　　处：《临床泌尿外科杂志》2005年第11期695-696,699,共3页Journal of Clinical Urology

基　　金：国家自然科学基金资助(基金编号:30271303);国家杰出青年科学基金资助(基金编号:30225046)

摘　　要：目的:探讨腺病毒介导人IL-2基因转染鼠膀胱移行上皮细胞及其表达的可行性,为膀胱癌的免疫治疗提供实验依据。方法:通过膀胱灌注的方法将重组腺病毒AdhIL-2灌入大鼠膀胱内,分不同时间段获取大鼠膀胱黏膜组织,利用RT-PCR的方法检测hIL-2基因在膀胱移行上皮细胞中的表达。结果:腺病毒介导hIL-2在膀胱移行上皮细胞中得到有效表达,基因表达持续时间达到7天。结论:腺病毒是一种有效介导基因的工具,hIL-2在膀胱上皮细胞中的持续表达为膀胱癌免疫治疗提供新的方法。Objective： To study the method of adenovirus mediated human IL-2 gene transfer to rat bladder transitional epithelium and the expression of the targeted gene. Methods： Gene transfer to rat bladder epithelium was accomplished via direct intravesical instillation using a replication-defective adenoviral vector containing hlL-2 gene. Successful gene transfer was confirmed by RT-PCR. Results：The hlL 2 gene was expressed successfully in rat bladder transitional epithelium, gene expression was evident at least 7 days. Conclusions： Adenovirus mediated gene transfer has potentially powerful applicability in the therapy of malignant diseases of the bladder.

关 键 词：腺病毒 HIL-2 基因转染 膀胱 

分 类 号：R511.8[医药卫生—内科学]