空肠弯曲菌HSP60家族成员的鉴定  

IDENTIFICATION OF A MEMBER OF HSP60 FAMILY IN CAMPYLOBACTER JEJUNI

作　　者：臧星星[1] 马宝骊[1] 王利[1] 柏峻[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所

出　　处：《中国免疫学杂志》1996年第2期72-74,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金;卫生部;上海市高教局与卫生局资助

摘　　要：用针对ＨＳＰ６０保守区的二株单抗ⅡＨ９和ＭＬ－３０进行了Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ和ＥＬＩＳＡ试验，发现空肠弯曲菌ＣＪ－Ｓ１３１株４３ｋＤ通道蛋白属ＨＳＰ６０家族成员，命名其为空弯菌ＨＳＰ４３。ＳＤＳ－ＰＡＧＥ和薄层扫描显示ＨＳＰ４３是主要菌体蛋白。虽然ＨＳＰ极端保守，但用ＣＪ－Ｓ１３ｌｌｙｓａｔｅ免疫小鼠后，发现最早诱导的抗体是针对ＨＳＰ４３的，并且这种抗体活性在随后８０ｄ的观察期间一直维持较高活性。此结果为研究感染诱致自身免疫病分子机理提供了新的线索。With monoclonal antibodies ⅡH9 and ML-30 which recognized evolutionarily conserved sequences of heat shock protein 60(HSP60)family,Western blotting and ELISA showed that 43kD outer membrane protein(porin)of campylobacter jejuni,strain CJ-S131,was a member of HSP60 family,termed the“HSP43”of campylobacter jejuni. The HSP43 was major component of CJ-S131 detected by SDS-PAGE and chromatogram scanning, Although HSP were among the most conserved proteins,the serum specific antibody against HSP43 appeared earliest and activated highly in experimental mice immunized with CJ-S131 lysate. Results provided a new way for further research on molecular mechanism of infection induced autoimmune diseases.

关 键 词：空肠弯曲菌 热休克蛋白 自身免疫 HSP60 鉴定 

分 类 号：R372[医药卫生—病原生物学]