严重烧伤后内皮细胞损伤及其在早期脏器损害发病中的作用  

THE EFFECT OF VASCULAR ENDOTHELIAL CELL ON THE PATHOGENESIS OF POSTBURN ORGAN DAMAGE

作　　者：杨宗城[1] 罗向东[1] 高建川[1] 吴雄飞[1] 李志清[1] 罗勤[1] 

机构地区：[1]第三军医大学西南医院

出　　处：《解放军医学杂志》1996年第1期10-12,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：３０％Ⅲ度烧伤兔伤后血浆渗透压下降、ＣＥＣ及ＡＣＥ活性升高，血管内皮细胞明显形态学改变，表明伤后内皮细胞受损。缺氧、烧伤血清、ＴＮＦ、内毒素、ＦＰＡＢ及活化粒细胞能损伤培养的人脐静脉内皮细胞。３０％Ⅲ度烧伤大鼠伤后血浆ＥＴ－ｌ明显增多，ＥＴ－１、ＥＴＡ、ＥＴＢ、ｍＲＮＡ于心肺肝肾的表达，于伤后斑点杂交与原位杂交的结果，均表明其明显增强。烧伤内皮素增加，不但使血管收缩，而且增加内皮细胞通透性。采用细胞微管吸吮技术系统，发现烧伤血清作用于粒细胞或人脐静脉内皮细胞，均使ＰＭＮＥＣ粘附力持续升高，加ＡＣＤ１１ａＣＤ１８单抗使之降低。A series of in vivo and in vitro studies was performed to investigate the vascular endothelial cell damage and the change of endothelin after burn injury. The results were as follow:vascular endothelial cells were darmaged markedly in those rabbits with 30% burn injury. Human umbilical vein or pulmonary arterial endothe- lial cells cultured in hypoxic environment with 4. 419%O2 or with burn serum, endotoxin, TNF were injured ,evi- denced by the increased LDH activity and the content of 6-keto-PGF la in medium ,the decreased content of G-actin and obvious pathologic changes in these cells。 The contents of endothelin in plasma and cardiac, hepatic, lung and kidney tissue increased significantly on rats with 30% burn injury. It was also found that the mRNA expression of ET-1 evidently increased. The results of localization of mRNA experessing cell by in situ hybridization demonstrat- ed the significant increase of mRNA of ET-1,ETA, ETB expression in all organs, The adhesion force between a single PMN and HUVEC pair increased if the EC or PMN incubated with burn sera, and it was reduced when CD11a/CD18mAbandCD11a/CD18mAbwere usd.

关 键 词：内皮细胞 内皮素 烧伤 脏器损害 

分 类 号：R644[医药卫生—外科学]