大鼠孤束核内H_3受体对哮喘发作时脑组织中Fos蛋白表达的影响  

Effect of histamine H_3 receptors in nucleus tractus solitarius on c-Fos expression in the brain of rats with asthma onset

作　　者：胡春梅[1] 董榕[2] 刘保森[2] 张晓红[2] 季吉[2] 

机构地区：[1]南京胸科医院六病区,江苏南京210029 [2]东南大学基础医学院生理学与药理学系,江苏南京210009

出　　处：《东南大学学报（医学版）》2005年第6期371-374,共4页Journal of Southeast University(Medical Science Edition)

基　　金：铁道部科技基金资助项目(4247600045)

摘　　要：目的:探讨大鼠孤束核(NTS)内组胺H3受体对哮喘发作时大脑Fos蛋白表达的影响及其意义。方法:取健康雄性SD大鼠制备哮喘模型并诱发哮喘发作,采用中枢立体定位及微量注射技术,分3组分别在NTS内微量注射无菌生理盐水、H3受体激动剂R--αmethylhistamine(RMHA)以及H3受体拮抗剂thioperamide(Thio)加RMHA,用SABC免疫组织化学方法测定大鼠哮喘急性发作后1 h Fos蛋白在丘脑至延髓平面各核团的分布情况。结果:与哮喘组相比,哮喘大鼠NTS内微量注射1μg RMHA,丘脑室旁核、下丘脑室旁核、室周核、NTS等核团Fos蛋白表达减少,阳性细胞数降低,差异有显著性(P<0.01);而用Thio 5μg预处理则可拮抗RMHA的上述效应。结论:NTS内H3受体可能通过丘脑室旁核、下丘脑室旁核和室周核等脑区相关神经元参与对哮喘的神经调控,从而可能抑制哮喘发作。Objective To explore the effect of histamine H3 receptors in nucleus tractus solitarius（NTS） on c-Fos expression in the brain of rats with asthma onset. Methods Male Sprague-Dawley（SD） rats were sensitized with ovalbumin to induce asthma using central stereotaxic and microinjection techniques. The model rats were randomly divided into three groups, injected NS, R-α-methylhistamine（RMHA, a H3 receptor receptors agonist）, and thioperamide（Thio, a H3 receptors antagonist） ＋ RMHA into NTS respectively. Fos positive ceils distributed in different nucleus were detected by immunohistochemistry SABC method after the challenge one hour later. Results After microinjection 1 μg RMHA into NTS at the onset of asthma, the number of Fos positive cell and Fos density presented in NTS, periventricular hypothalamic nucleus（Pe）, hypothalamic paraventricular nucleus（Pa）, and raraventricular thalamic nucleus（PVN） decreased markedly （ P 〈 0.01）. These effects can be blocked by pre-treatment with 5 μg microinjection of Thiop into NTS. Conclusion This study suggests that histamine H3 receptors in NTS can ameliorate asthma via regulating some neurons in Pa, Pe and PVN.

关 键 词：大鼠 孤束核 H3受体 哮喘 FOS蛋白 表达 

分 类 号：R562.25[医药卫生—呼吸系统]