LDL及HDL对内皮细胞血栓调节蛋白mRNA表达的影响  

The Effects of LDL and the Expressions of Thrombomodulin mRNA in Endothelial Cells

作　　者：王伟成[1] 董晓峰[1] 周凤鑫 经建中 黄桂秋[1] 徐也鲁[1] 

机构地区：[1]上海第二医科大学病理生理学教研室

出　　处：《上海第二医科大学学报》1996年第3期166-168,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金

摘　　要：研究ＬＤＬ及ＨＤＬ对血管内皮细胞血栓调节蛋白ｍＲＮＡ表达的影响。利用ＲＴ－ＰＣＲ技术的敏感性，研究分析在各种浓度ＬＤＬ及ＨＤＬ刺激下对牛内皮细胞血栓调节蛋白ｍＲＮＡ表达的影响。结果表明，ＬＤＬ能使ＴＭ－ｍＲＮＡ表达上升，ＬＤＬ和ＨＤＬ浓度的变化对ＴＭ－ｍＲＮＡ的表达均没有影响，而ＨＤＬ与正常对照组相比无明显变化，但ＨＤＬ具有抑制ＬＤＬ促使ＴＭ－ｍＲＮＡ表达的作用。The purpose of this paper is to study the effects of LDL and HDL on the expression of thrombomodulin (TM) mRNA in bovine endothelial cells. We investigated the effect of different kinds of LDL and HDL in various concentrations,by using the sensitivity of RT-PCR. It showed that LDL increased the levels of TM mRNA expression but without a dose-dependent pattern while HDL had no effect.It also showed that HDL inhibted the up-regulated effects of LDL on TM mRNA expressions while endothelial cells were incubated with the both lipoproteins together.

关 键 词：低密度 脂蛋白 高密度 内皮细胞 血检调节蛋白 

分 类 号：Q513.5[生物学—生物化学]