γ-羟基丁酸对神经细胞缺氧复氧损伤的保护作用与γ-氨基丁酸及其A受体的关系  

Relationship between GHBA' s protective effect on hypoxia-reoxygenation neuronal damage and GABA and GABA_4receptor

作　　者：赵庆杰[1] 刘玉梅[2] 宋冬晶[3] 郜旭[1] 苏志强[1] 

机构地区：[1]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001 [2]黑龙江省医院,黑龙江哈尔滨150036 [3]吉林大学中日联谊医院,吉林长春130023

出　　处：《中风与神经疾病杂志》2005年第5期416-418,共3页Journal of Apoplexy and Nervous Diseases

基　　金：黑龙江省科技攻关计划项目(No.GB02C 137-02)

摘　　要：目的探讨γ-羟基丁酸(GHBA)对大鼠皮层神经元缺氧复氧损伤的保护作用与γ-氨基丁酸(GABA)和GABA受体A型α1亚型(GABAARα1)阳性神经元表达的关系。方法采用原代培养新生大鼠皮层神经元缺氧复氧损伤模型,分为缺氧前GHBA干预组、缺氧后GHBA干预组和未干预组．于复氧24h后,观察细胞存活数、GABA和GABAARα1阳性神经元的表达。结果(1)GHBA干预各组皮层神经元细胞存活数、GABA和GABAARα1阳性细胞数均高于相应未干预组(P<0．05)。(2)缺氧前GHBA干预组细胞存活数、GABA和GABAARα1阳性细胞表达数均高于相对应缺氧后干预组(P<0．05)。结论GHBA可通过上调GABA和GABAARα1阳性神经元的表达水平对缺氧复氧皮层神经元发挥保护作用。Objective To investigate relationship between GHBA＇s protective effect on hypoxia-reoxygenation neuronal damage and GABA and GABAARα1 positive neurons. Methods Make hypoxia-reoxygenation damage model on newborn rat cortical neurons,which were randomly devided into three groups ：intervention A group, intervention B group and non-intervention group. Every group had 24 hours＇ reoxygenation,observed at different time. Results （1）Survival neurons,GABA and GABAARα1 positive neurons in intervention groups were more than non-intervention group （P〈0.05）. （2）Survival neurons ,GABA and GABAARα1 positive neurons in intervention A group were more than non-intervention group（P〈0.05）, Conclusion GHBA can protect hypoxia-reoxygenation neurons through upregulating GABA and GABAARα1 positive neurons.

关 键 词：GHBA 皮层神经元 缺氧复氧损伤 GABA GABAARα1 

分 类 号：Q591.2[生物学—生物化学]