谷氨酸对大鼠小脑脑片间位核神经元放电的影响  

Effect of glutamate on neuronal discharge of interpositus nucleus in rat cerebellar slices

作　　者：马颂华[1] 李冰[1] 邱一华[1] 

机构地区：[1]南通大学基础医学院生理学教研室,南通226001

出　　处：《南通大学学报（医学版）》2005年第5期316-318,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省高校自然科学研究重点项目(04KJA180110);南通市社会发展项目(S40057)

摘　　要：目的:应用细胞外记录单位放电技术,在离体大鼠小脑脑片上观察谷氨酸对间位核神经元单位放电的影响。方法:制备大鼠小脑脑片,细胞外记录小脑间位核神经元单位放电,并观察谷氨酸对大鼠小脑间位核神经元单位放电的影响。结果:谷氨酸对小脑间位核神经元放电有兴奋作用,且这种兴奋作用不能被低Ca2+/高Mg2+的人工脑脊液所阻断。结论:谷氨酸对小脑间位核神经元单位放电的兴奋作用可能是通过直接激活间位核神经元表面的谷氨酸受体实现的。Objective：The effect of glutamate on the discharge of neurons in cerebellar interpositus nucleus was investigated. Methods ：The cerebellar slices were prepared and spontaneous discharges of single cerebellar interpositus nuclear neurons were recorded by using extracellular recording technique. Results ：Glutamate evoked a significant increase in firing rate of the cerebellar interpositus nuclear neurons. A low - Ca^2＋/high - Mg^2＋ medium did not block the excitatory response of cerebellar interpositus nuclear neurons to glutamate. Conclusion：The excitatory effect of glutamate on the discharge of interpositus nuclear neurons in cerebellar slices may be directly mediated by glutamate receptors on interpositus nuclear neurons.

关 键 词：小脑脑片 间位核 谷氨酸 

分 类 号：R338.23[医药卫生—人体生理学]