α_2-肾上腺素受体在T淋巴细胞中的表达  

Expression of α_2-adrenoreceptor mRNA in T lymphocytes of rats

作　　者：包璟崟[1] 曹蓓蓓[1] 邱一华[1] 

机构地区：[1]南通大学基础医学院生理学教研室,南通226001

出　　处：《南通大学学报（医学版）》2005年第5期319-320,323,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省高校自然科学研究重点项目(04KJA180110);南通市社会发展项目(S40057)

摘　　要：目的:从分子水平揭示T淋巴细胞表达α2-肾上腺素受体(α2-AR)mRNA的现象,为神经内分泌系统调节免疫功能的机制提供更多新的证据。方法:取大鼠肠系膜淋巴结细胞,用尼龙毛柱粘附法分离和纯化T淋巴细胞,应用E花环法检测T细胞的分离纯度,用逆转录-聚合酶链反应(RT-PCR)法检测T细胞中α2-AR mRNA的表达。结果:经过对肠系膜淋巴结细胞的分离和纯化,获得的T细胞的纯度明显高于未分离和纯化的淋巴细胞,但分离纯化后的T细胞,其活性仍然保持与分离前T细胞的活性相似;这些纯化的T细胞可表达α2-AR mRNA。结论:淋巴细胞可能像机体的其它组织细胞一样能表达和合成α2-AR。Objective： Our previous work has shown that α2-and β - adrenoreceptors exist on surface of lymphocytes of rats from their functional profiles. At present,however, there is no direct evidence to show the expression and synthesis of the subtypes of α2-adrenoreceptors by lymphocytes . We therefore investigated the expression of α2- adrenoceptor （α2- AR ） mRNA in T lymphocytes in order to provide more evidence for the modulation of lymphocyte function by neuro - endocrine system. Methods：Lymphocytes were separated from mesenteric lymph nodes of rats. T cells were purified by passing through a nylon wool column. Then the purity and the viability of T lymphocytes were determined with E - rosette forming test and trypan blue exclusion, respectively. Reverse transcription-polymerase chain reaction （RT- PCR） was used to measure the expression of α2-AR mRNA. Results ：The purity of T ceils that were separated was significantly higher than that of T ceils that were not separated, but these T ceils separated remaines smilar to those not separated in viability. These purified T ceils expressed α2-AR mRNA. Conclusion：T lymphocytes may express and synthesize α2-AR as neurons and endocrine cells do.

关 键 词：T淋巴细胞 α2-肾上腺素受体 逆转录-聚合酶链反应 

分 类 号：R33[医药卫生—人体生理学]