高效液相色谱-在线消解-氢化物发生原子吸收光谱联用技术研究  

High Performance Liquid Chromatography Combined with Atomic Absorption Spectrometry Using Ultraviolet Digestion-Hydride Generation Interface

作　　者：刘华琳[1] 赵蕊[1] 韦超[1] 邢志[1] 闫继仁[2] 张新荣[1] 

机构地区：[1]清华大学化学系原子分子纳米科学教育部重点实验室 [2]中国环境科学研究院,北京100012

出　　处：《分析化学》2005年第11期1522-1526,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20375022);科技部(No.2002ba906a28)资助项目

摘　　要：运用自行设计的接口,实现了高效液相色谱与原子吸收光谱的联用。通过对常见砷化合物进行形态分析,考察了自行设计的高效液相色谱-紫外在线消解-氢化物发生原子吸收光谱联用(HPLC-UV-HGAAS)接口的性能。实现了将分离后不能直接用于氢化物发生的大分子,通过紫外“在线”消解成小分子砷化合物的目的。确定了仪器的最优化分析条件。建立了快速、直接、连续、在线的HPLC-UV-HGAAS元素形态分析方法。The coupling of high performance liquid chromatography （HPLC） with atomic absorption spectrometry （AAS） was achieved by using a simple laboratory-built ultraviolet （UV）-hydride generation （HG） interface. Compared with the former techinque, a 0.4 m quartz tube was used for UV digestion instead of the 5 m teflon coil without decreasing the UV digestion efficiency of the organic arsenic compounds. As a nesult, the volume of the interface was greatly minished, so the miniaturization and integration of the instrument was achieved . The coupling instrument was used to separate four arsenic species （ arsenite , arsenate , methylar - sonic acid, dimethylarsinic acid ） that are of toxic importance. The optimized analysis conditions were obtained. The results showed good repeatability （ RSD 〉 6.56% ）, good linearity （ r 〉 0. 997 ） and low detection （ detection limit 〉 14.4 mg/L）. Then the performance for on-line digestion of organic arsenic species including arsenobetaine （AsB） and arsenocholine （AsC） was investigated. A rapid, direct, continuous, online HPLC-UV-HGAAS hyphenated technique was developed.

关 键 词：高效液相色谱 原子吸收光谱 联用技术 元素形态分析 仪器接口 砷 氢化物发生 在线 消解 形态分析方法 

分 类 号：O657.31[理学—分析化学] O657.72[理学—化学]