全内反射荧光光谱法研究水溶性卟啉在正己烷/水界面的吸附行为  

Adsorption Behavior of a Water-soluble Porphyrin at n-Hexane/Water Interface by Total Internal Reflection Fluorescence

作　　者：黎朝[1] 唐尧基[1] 陈莹[1] 陈静怡[1] 李海燕[1] 李耀群[1] 

机构地区：[1]厦门大学化学化工学院化学系现代分析科学教育部重点实验室,厦门361005

出　　处：《分析化学》2005年第11期1543-1546,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.29875023);福建省自然科学基金资助项目(No.B0410002)

摘　　要：在自行组装的全内反射荧光测定装置上实现了液/液界面全内反射荧光光谱的测绘,比较了水溶性的m eso-四(对磺酸基苯基)卟啉(TPPS)在正己烷/水界面上与在水相中荧光性质的差异,研究了全内反射荧光强度随表面活性剂种类、浓度及溶液pH值的变化情况,探索了TPPS的界面吸附行为,着重考察了阳离子表面活性剂CTMAB对TPPS界面荧光性质的影响。结果表明,在阳离子表面活性剂存在的条件下,未质子化的TPPS能够选择性地吸附在正己烷/水界面上,静电力在TPPS界面吸附过程中应起重要作用。Liquid/liquid total internal reflection fluorescence （TIRF） spectra measurements were realized on a home-made spectrofluorimeter. A comparison was made for the differences in fluorescence spectra of mesotetrakis （4-sulphonatophenyl） porphyrin （ TPPS ） at n-hexane/H2O interface and in aqueous solution by using TIRF. It was indicated that deprotonated forms of TPPS （TPPS^4- ） were adsorbed selectively at the n-hexane/ H2O interface in the presence of cationic surfactants. The adsorption behavior of TPPS under different concentrations and kinds of surfactants were studied. It showed that electrostatic interaction had important effect on the adsorption of TPPS at liquid/liquid interface.

关 键 词：液/液界面 meso-四(对磺酸基苯基)卟啉 全内反射荧光 水溶性卟啉 荧光光谱法 全内反射 吸附行为 水界面 正己烷 阳离子表面活性剂 

分 类 号：O657.3[理学—分析化学] O647.31[理学—化学]