天然水体中痕量微囊藻毒素的高效液相色谱测定方法优化  

The Optimization of High Performance Liquid Chromatographic Method for Analysis of Trace Microcystins in Natural Water Bodies

作　　者：刘碧波[1] 肖邦定[1] 刘剑彤[1] 方涛[1] 刘永定[1] 

机构地区：[1]中国科学院水生生物研究所

出　　处：《分析化学》2005年第11期1577-1579,共3页Chinese Journal of Analytical Chemistry

基　　金：中国科学院重大项目(No.KZCX1-SW-12);国家973项目(No.2002CB412300);中国科学院水生物研究所创新课题(No.220316)资助项目

摘　　要：以微囊藻毒素LR(MC-LR)和微囊藻毒素RR(MC-RR)为材料,主要通过调节杂质淋洗液和毒素洗脱液中甲醇、水和三氟乙酸(TFA)的配比来改变极性和PH值,对高效液相色谱(HPLC)法分析MC环境样品的方法进行了优化.结果表明,含0.1%TFA的40%～45%的甲醇水溶液可以取得较好的杂质淋洗效果;含0.1%TFA 的70%的甲醇水溶液可以将固相萃取柱(SPE)上的MC完全洗下.因此,建议在分析杂质较多的环境样品时,使用含0.1%TFA的40%～45%的甲醇水溶液对杂质进行淋洗,然后用含0.1%TFA的70%～100%甲醇水溶液对MC进行洗脱.The ratio of methanol, water and trifluoroacetic acid （TFA） was regulated to change the polarity and the pH of the rinse solution and the eluent, so as to improve the high performance liquid chromatography （HPLC） detection method for trace microcystines （MCs） in natural water bodies. The results showed that 40% -45% methanol-water solution containing 0.1% TFA could get good effects on the rinse of impurity ,and 70% methanol-water solution containing 0.1% TFA could elute all the MCs in solid phase extraction （SPE）cartridge （C18）. In this way, it is suggested that, in analysis of environmental samples with high concentration of impurity, impurity should be washed with 40% -45% methanol-water solution containing 0.1% TFA, and MCs should be eluted with 70% -100% methanol-water solution containing 0.1% TFA.

关 键 词：微囊藻毒素 高效液相色谱 天然水体 高效液相色谱(HPLC) 测定方法 优化 甲醇水溶液 痕量 环境样品 固相萃取柱 

分 类 号：O657.72[理学—分析化学] X832[理学—化学]