壳聚糖/聚乙烯吡咯烷酮固定辣根过氧化物酶的过氧化氢生物传感器  

Hydrogen Peroxide Biosensor Based on the Immobilization of Horseradish Peroxidase in Chitosan/Polyvinylpyrrolidone Hybrid Film

作　　者：王怀生[1] 潘芊秀[1] 王桂香[1] 

机构地区：[1]聊城大学化学化工学院分析化学研究所,聊城252059

出　　处：《分析化学》2005年第11期1623-1626,共4页Chinese Journal of Analytical Chemistry

基　　金：山东省自然科学基金资助课题(No.Y96B13032)

摘　　要：用一种新型的壳聚糖（CS）／聚乙烯吡咯烷酮（PVP）复合膜在玻碳电极（GCE）上固定辣根过氧化物酶（HRP）。以乙-醛作交联剂，二茂铁（Fc）作媒介体，制备过氧化氢生物传感器。红外光谱表明：CS与PVP交联形成了一种新的高聚物，实验结果证明该聚合物适合辣根过氧化物酶的固定。该传感器对于H2O2的电流响应在58内即可达到最大，线性范围为6．0×10^-6～1．7×10^-4moL／L；检出限为2．5×10^-6moL／L。该传感器的检测灵敏度为62．5μA／mmoL／L。A new type of chitosan （CS）/polyvinylpyrrolidone （PVP） hybrid composite material was developed for the fabrication of an amperometric H2O2 biosensor based on the immobilization of horseradish peroxidase （HRP） in the chitosan/PVP hybrid film. Glyoxal was selected as a crosslinker and ferrocene as a mediator. The results of infrared （IR） spectra suggest that a new polymer was produced when mixed CS with PVP. The biosensor had a fast response of less than 5 s to hydrogen peroxide and the catalytic currents have a linear relationship with the H202 concentration in the range of 6.0 × 10^-6 ～ 1.7 × 10^-4 mol/L, with a detection limit of 2.5 × 10^ -6 mol/L based on S/N= 3. The sensitivity of this biosensor was 62.5 μA（mmol/L） - Keywords Chitosan, polyvinylpyrrolidone, horseradish peroxidase, hydrogen peroxide

关 键 词：壳聚糖 聚乙烯吡咯烷酮 辣根过氧化物酶 过氧化氢 聚乙烯吡咯烷酮(PVP) 过氧化氢生物传感器 检测灵敏度 玻碳电极 红外光谱 电流响应 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]