反义RNA技术及其在农业领域的应用  

Antisense RNA Technology and Its Application in Agricultural Field

作　　者：娄虹[1] 杨淑琴[1] 

机构地区：[1]辽宁大学环境与生命科学学院,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2005年第4期328-333,共6页Journal of Liaoning University：Natural Sciences Edition

摘　　要：介绍了反义RNA的概念及作用机理,对反义RNA技术及其在农业领域的应用进行了概述.反义RNA技术是借助基因重组技术,根据碱基互补原理,用人工合成或生物体合成的特定RNA片段(或其化学修饰物)抑制或封闭基因表达的技术.在农业领域反义RNA技术主要应用于果实性状及品质的调控,对观赏植物性状的调控,植物抗病,控制油料种子中脂肪酸的合成,控制雄性不育,降低或去除食物中有害化学成分等方面其功能获得可靠的成效.This paper presents the definition and action mechanism of antisense RNA; Besides, the paper reviews antisense RNA technology and its application in agricultural field. The antisense RNA technology, based on the principle of base complementation, is a method using specific complementary nucleotide fragments to target RNA for inhibition or disclosing of gene expression. The primary application of antisense RNA technology in agricultural field includes： adjusting and controlling fruit character; controlling ornamental plant character; resisting diseases ; controlling the synthesis of fatty acids in the oilseed crops ; controlling male sterile; reducing or being apart from harmful chemical composition of food and so on.

关 键 词：反义RNA 反义RNA技术 基因调控 

分 类 号：Q522[生物学—生物化学]