高效液相色谱法测定聚甲酚磺醛溶液中间甲酚-6-磺酸二聚体含量  

Determination of m-cresol-6-sulfonic acid ammonium dimmer in policresulen solution by HPLC

作　　者：徐家根[1] 初立梅[1] 陆继好 

机构地区：[1]南京医科大学附属南京儿童医院,江苏南京210008 [2]南京信业医药技术有限公司,江苏南京210014

出　　处：《中国医院药学杂志》2005年第5期437-438,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的用高效液相色谱法测定聚甲酚磺醛溶液中间甲酚6磺酸二聚体的含量。方法采用ODS色谱柱(ShimpackCLCODS,150mm×6mm,5μm),流动相为甲醇1%醋酸铵水溶液(40∶60),检测波长为280nm,流速为1.0mL·min-1。采用外标法测定间甲酚6磺酸二聚体的含量。结果间甲酚6磺酸铵二聚体在8.24～98.88mg·L-1浓度范围内线性关系良好,r=0.9995。结论本方法快速、可靠、简单、灵敏,适用于该制剂的质量分析检验。OBJECTIVE A method of the determination of m-cresol-6-sulfonic acid ammonium dimmer in policresulen solution by HPI.C was established. METHODS An ODS column（ 150mm×6mm,5μm） was used. The mobile phase was methanol-1 % ammonium acetate（40：60）, the detectiVE wavelength was 280 nm and the flow rate was 1. 0 mL·min^-1 , the content was calculated using external standard method. RESULTS The calibration curve was linear in the range of 8. 24 - 98. 88mg·L^-1 （r = 0. 999 5）. CONCLUSION This method is simple and accurate for quantitative analysis of the preparaTion.

关 键 词：聚甲酚磺醛溶液 间甲酚-6-磺酸二聚体 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]