高效液相色谱法测定愈心痛胶囊中延胡索乙素含量  

Determination of tetrahydropalmatine content in Yuxintong capsules by HPLC

作　　者：薛连海[1] 丁斌[1] 何建中[1] 杨伟祥[2] 

机构地区：[1]吉林化工学院,吉林吉林132022 [2]吉林敖东集团大连药业有限公司,辽宁大连116014

出　　处：《中国医院药学杂志》2005年第5期443-444,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的建立愈心痛胶囊质量控制的新方法。方法采用甲醇萃取愈心痛胶囊中的延胡索乙素,高效液相色谱法测定愈心痛胶囊中延胡索乙素的含量。色谱柱为HypersilBDSC18(4.6mm×200mm,5μm),流动相为甲醇磷酸盐溶液(67∶33,磷酸二氢钾0.1g和磷酸氢二钾4.8g混合溶于500mL水中),流速为1mL·min-1,检测波长为282nm。结果外标曲线回归方程为Y=2.75+0.18X,r=0.9997。平均回收率为99.8%。RSD为1.3%(n=5)。结论方法简便、快速,重现性好,可用于愈痛胶囊的质量控制。OBJECTIVE A new method for the quality control of Yuxintong capsules was established. METHODS Tetrahydropalmatine in Yuxintong capsules was extracted using methanol as extractant, and then the tetrahydropalmatine content was determined by HPLC. Using Hypersil BDS C,ucolumn（4.6mm×200mm,5μm）. Methanol-phosphate solution （dissolved 0. 1 g potassium dihydrogen phosphate and 4. 8 g potassium hydrogen phosphate with 500mL water ） （67：33） was used as mobile phase. The flow rate was 1.0 mL·min^-1. Detective wavelength was 280 nm RESULTS average recovery was 99.8 % and RSD was 1.3 %. CONCLUSION sules. The regression equation was Y = 2. 75 ＋0. 18X,r= 0. 999 7. The The method can be used for the quality control of Yuxintong capsules.

关 键 词：愈心痛胶囊 延胡索乙素 高效液相色谱法 含量测定 

分 类 号：R272.2[医药卫生—中医儿科学]