毛囊bulge细胞在角膜缘基质诱导下向角膜上皮细胞分化的初步研究  

Transdifferentiation of hair follicle bulge cells into corneal epithelial cells induced by corneal limbal stroma in vitro

作　　者：余瑾[1] 杨珂[1] 杨恬[1] 符刚[1] 

机构地区：[1]第三军医大学基础医学部细胞生物学教研室,重庆400038

出　　处：《第三军医大学学报》2005年第20期2016-2019,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30371383);重庆市攻关项目(2004796)~~

摘　　要：目的探讨体外培养的毛囊bulge细胞向角膜上皮细胞分化的可能性。方法体外分离培养毛囊bulge细胞,然后在transwell中与角膜缘基质细胞共培养诱导分化,观察毛囊bulge细胞的分化情况,免疫组化检测K12及K19表达的变化。结果体外培养的毛囊bulge细胞保持高增殖,低分化状态。经过2周左右的共培养,毛囊bulge细胞逐渐分化,部分细胞K12表达阳性。结论角膜缘基质细胞可以诱导毛囊bulge细胞向角膜上皮细胞分化。Objective To investigate the feasibility of the transdifferentiation of hair follicle bulge cells into corneal epithelial cells in vitro. Methods The hair follicle bulge cells from 7- to 8-day SD rats were isolated and cultured in vitro, then co-cultured with rabbit corneal limbal stroma in transwell-cuhured system. The dif- ferentiation and development of hair follicle bulge cells were observed, and immunocytochemical staining were used to detect expression of K19 and K12 in hair follicle bulge cells. Results The cultured bulge cells possesed high proliferation and low differentiation, co-expressed K19 and β1 integrin, but part of them expressed K12 after 2-week co-culture with rabbit corneal limbal stroma in transwell-cuhured system. Conclusion Rat hair follicle bulge cells could transdifferentiate into corneal epithelial cells induced by corneal limbal stroma in vitro.

关 键 词：毛囊 bulge细胞 角膜 上皮 转分化 

分 类 号：R322.91[医药卫生—人体解剖和组织胚胎学] R322.99[医药卫生—基础医学]