电针对佐剂性关节炎大鼠下丘脑CRH、IL-2、β-EP含量的影响  

Effects of electroacupuncture on CRH, IL-2 and β-EP contents in the hypothalamus in the rat of adjuvant arthritis

作　　者：李辉[1] 李晓泓[2] 张露芬[2] 朱文莲[2] 翟景慧[2] 周登芳[2] 

机构地区：[1]卫生部中日友好医院针灸科,北京100029 [2]北京中医药大学针灸学院

出　　处：《中国针灸》2005年第11期793-796,共4页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金课题:90209026;教育部优秀青年骨干教师科研资金资助

摘　　要：目的:探讨电针抗炎免疫调节作用机理,比较“大椎”“命门”穴与非穴间的作用差异。方法:以佐剂性关节炎大鼠(AA)为研究对象,随机分为5组:正常组、模型组、电针“大椎”组、电针“命门”组、电针非穴组。观察电针对关节炎症局部及下丘脑促肾上腺皮质激素释放激素(CRH)、β-内啡肽(βEP)、白细胞介素-2(IL-2)含量的影响,并比较不同穴位间的作用差异。结果:电针“大椎”组下丘脑CRH的含量较模型组降低(P<0.05),电针各组下丘脑β-EP、IL-2的含量与模型组比较差异无显著意义(P>0.05);而下丘脑CRH含量与IL2含量,下丘脑IL-2含量与βEP含量具有显著正相关关系(γ=0.886,γ=0.946)。“大椎”组、“命门”组足爪肿胀率较非穴组低(P<0.05)。结论:电针可能是通过下丘脑CRH、IL-2、βEP等因素的相互调节,起到抗炎免疫调节作用的。“大椎”“命门”抗炎作用优于非穴处。Objective To explore Mechanisms of electroacupuncture in anti inflammation and immunoregulation, and to compare differences among “Dazhui”（GV 14）, “Mingmen ”（GV 4） and non-acupoint in these actions. Methods Adjuvant arthritis rat model was made. The rats were divided into 5 groups： normal group, model group, “Dazhui” （GV 14） electroacupuncture group, “Mingmen” （GV 4） electroacupuncture group and non-acupoint electroacupuncture group. Effects of electroacupuncture on local joint inflammation and corticotropin-releasing hormone （CRH）, β-endorphin （β-EP） and interleukin-2 （IL-2） contents in the hypothalamus were observed and differences of the actions among different points were compared. Results CRH content in the hypothalamus in the “Dazhui” （GV 14） electroacupuncture group decreased as compared with the model group （P〈0.05）; there was no significant change in β-EP and IL-2 contents of the hypothalamus in the 3 electroacupuncture groups as compared with the model group （P〉0.05）; there was positive correlation between CRH and IL-2 contents, and between IL-2 and 13-EP contents in the hypothalamus. The swelling rate of the toe in the “Dazhui” （GV 14） group and the “Mingmen”（GV 4） group was lower than that in the non acupoint group （P〈0.05）. Conclusion Electroacupuncture exerts anti-inflammation and immunoregulation possibly via regulation of CRH, IL-2 and 13-EP each other, and the anti-inflammation action of “Dazhui” （GV 14） and “Mingmen”（GV 4） is stronger than that of non-acupoint.

关 键 词：关节炎 实验性/针灸疗法 电针 下丘脑/针灸效应 白细胞介素2/代谢 促肾上腺皮质 素释放激素/代谢 β内啡肽/代谢 

分 类 号：R245.97[医药卫生—针灸推拿学]