山楂水提液清除氧自由基作用的研究  

Study on the Eradicating Effect of Oxygen Free Radicals by Hawthorn

作　　者：龚建武 王晓春[2] 

机构地区：[1]湖南长沙市第一医院检验科,湖南长沙410005 [2]中南大学技术与情报学院检验系

出　　处：《实用预防医学》2005年第3期568-569,共2页Practical Preventive Medicine

摘　　要：目的探讨山楂抗氧自由基的作用。方法用邻二氮菲-Fe2+氧化法测定羟自由基(·OH);用改良的邻苯三酚氧化法测定超氧阴离子自由基(O2-·)。结果鲜山楂及干山楂水提液均有清除·OH及O2-·的作用,与对照管比较有高度显著性(P<0.01),鲜山楂水提液清除氧自由基能力大于干山楂水提液(P<0.01)。结论山楂水提液有较强清除氧自由基的作用。Objective To explore the scavenging effect of hawthorn on oxygen free radicals. Methods Hydroxyl radicals （·OH） produced in H2 O2/Fe^2＋. system can oxidize phenanthrolene-Fe^2＋ into phenanthrolene- Fe^3＋ , so the extant of · OH activity was measured by method of phenanthrolene- Fe2^2＋ oxidation; and the superoxide anion radicals （O2^- ） activity was measured by improved method of pyvogallol auto oxidation. Results The scavenging effects of fresh hawthom on · OH and O^2- were both significant（P 〈 0.01）. Significant differences of antioxidative effect were observed between the fresh and dry hawthorn extracts （P 〈 0.01）. Conclusion Fresh and dry hawthorn water extracts possess strong of oxygen free radicals.

关 键 词：山揸 羟自由基 超氧阴离子自由基 分光光度法 

分 类 号：R961[医药卫生—药理学]