毛细管电泳高频电导法测定制剂中卡托普利  

CAPILLARY ELECTROPHORESIS-HIGH FREQUENCY CONDUCTIMETRIC DETERMINATION OF CAPTOPRIL IN PHARMACEUTICALS

作　　者：林伟丰[1] 吕宇轩[1] 陈缵光[2] 蔡沛祥[1] 莫金垣[1] 

机构地区：[1]中山大学化学与化学工程学院,广州510275 [2]中山大学药学院,广州510089

出　　处：《理化检验（化学分册）》2005年第11期796-798,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：广东省自然科学基金重点项目(021808);广东省教育厅科学基金项目(Z03055)

摘　　要：建立了制剂中卡托普利毛细管电泳高频电导分析法,并用于卡托普利片、复方卡托普利片中卡托普利含量的测定.对电泳介质的种类、浓度以及操作电压和进样量等影响因素进行了优化.试验采用3.0 mmol·L-1环己胺+5.0 mmol·L-1 H3BO3+0.50 mol·L-1乙醇作为电泳介质,20.0 kV为分离电压,可在 8min内实现对卡托普利的分离检测.卡托普利的线性范围为5.0～550 mg·L-1,检出限为0.8 mg·L-1,回收率达95.5%～102.0%.Capillary electrophoresis （CE） with conductimetric detection was applied to the determination of captopril, the main component of some drugs. Main conditions of analysis, i.e. kinds of electrophoretic medium and suitable concentration, working voltage, amount of sample introduced and etc, were studied and optimized. As a result, a mixed solution containing 3.0 mmol · L^-1 cyclohexylamine, 5.0 mmol· L^-1 H3BO3 and 0.50 mol · L^-1 ethanol was used as the electrophoretic medium, a separation voltage of 20. 0 kV was applied in CE, the time and height of sample introduction taken were 15. 0 s and 23.0 cm respectively. The separation and detection of captopril were completed within 8 min Under optimum experimental conditions, linear relationship in the range from 5. 0μg to 550μg per mL of captopril, with the value of RSD of 0. 8 mg · L^-1 and recoveries in the range of 95. 5% 102.0%, were obtained.

关 键 词：毛细管电泳 高频电导检测 非接触式电导检测 卡托普利 复方卡托普利片 

分 类 号：O657.8[理学—分析化学]