淫羊藿提取物对小鼠自发活动和睡眠功能的影响(英文)  

Effects of herba epimedium extract on spontaneous activity and sleep function of mice

作　　者：彭友霖[1] 朱修援[2] 曾靖[3] 黄贤华[3] 

机构地区：[1]赣南医学院数理教研室,江西省赣州市341000 [2]赣南医学院分析实验室,江西省赣州市341000 [3]赣南医学院药理教研室,江西省赣州市341000

出　　处：《中国临床康复》2005年第40期164-166,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:本草纲目记载小檗科淫羊藿属植物淫羊藿有益精气,坚筋骨,补腰漆,强心力等功能,近年来临床应用及药理作用报道较多。目的:观察在抖笼换能器法,阈上、阈下剂量戊巴比妥钠干预的情况下淫羊藿提取物对小鼠自发活动和睡眠功能的影响。单位:赣南医学院,数理教研室,分析实验室,药理教研室。材料:健康成年昆明种雄性小鼠110只,清洁级,体质量18～22g。方法:2004-08/09在赣南医学院科研中心实验室完成。①小鼠穴n=30雪腹腔注射1000g/L的淫羊藿提取物穴0.01mL/g雪,15min后放入吊笼中,稳定3min后记录用药后15s内自发活动波形。②小鼠穴n=40雪腹腔注射1000g/L的淫羊藿提取物穴0.01mL/g雪,15min后,腹腔注射阈上剂量的戊巴比妥钠穴2.5g/L雪0.02mL/g,观察小鼠翻正反射消失时间。③小鼠穴n=40雪腹腔注射1000g/L的淫羊藿提取物穴0.01mL/g雪,15min后,腹腔注射阈下剂量的戊巴比妥钠穴2.5g/L雪0.01mL/g,观察小鼠翻正反射消失时间。主要观察指标:小鼠自发活动次数;阈上剂量戊巴比妥钠小鼠睡眠时间;阈下剂量戊巴比妥钠小鼠睡眠时间。结果:实验动物110只,全部进入结果分析。①浓度为0.1mL/10g的淫羊藿提取物具有明显抑制小鼠自发活动,与对照组比较中波、大波出现次数显著减少(7.4±6.5,47.1±18.7;t=3.265,P<0.01)。②浓度为0.01mL/g淫羊藿提取物能显著加速阈上剂量戊巴比妥钠小鼠的入睡时间和延长睡眠时间,与对照组差异显著眼穴3.9±2.8雪,(219.7±87.2);穴5.8±2.9雪,(113.0±77.4)min;t=2.452,2.501,P<0.05演。③浓度为0.01mL/g淫羊藿提取物能显著加速阈下剂量戊巴比妥钠的入睡时间和延长睡眠时间,与对照组比较差异显著眼穴6.6±5.2雪,(60.3±71.7);0,0min;t=3.275,P<0.01演,与戊巴比妥钠有协同的中枢抑制作用。结论:淫羊藿提取物具有明显的中枢抑制作用,能明显抑制小鼠自发活动,同时还能明显加快阈上剂量戊�BACKGROUD： According to the record of Compendium of Herbology of herba epimedium, Berberidaceae barrenwort genus, herba epimedium has the effects of supplementing essence qi, consolidating bones and tendons, tonifying bruise of loin and strengthening cardiac ability, etc. In recent years, its clinical applications and pharmacological effects have been reported more frequently. OBJECTIVE： To investigate the effects of herba epimedium extract on spontaneous activity and sleep function of mice under the intervention of suprathreshold and subthreshold dose of pentobarbital sodium with the method of jiggle-cage transducer. SETTING： Department of Mathematics and Physics, Laboratory of Analysis, Department of Pharmacology, Gannan Medical College. MATERIALS： A total of 110 healthy adult Kunming male mice of clean grade, with a body mass between 18 g and 22 g. METHODS： The experiment was conducted in Laboratory of Scientific Research Institute of Gannan Medical College from August to September 2004. ① The mice （n=30） were injected intraperitoneally with 10130 g/L herba epimediu extract（0.01 mL/g） and put into hanging cage 15 minutes later, after a 3-minute stabilization period, forms of spontaneous activity within 15 s after the administration of drug were recorded. ②The mice （n=40） were intraperitoneally injected with 1000 g/L herba epimediu extract（0.01 mL/g）, 15 minutes later, injected intraperitoneally with 0.02 mL/g suprathreshold dose of pentobarbital sodium （2.5 g/L）, and then observe the disappearance time of righting reflex of the mouse. ③ The mice （n=40） were injected intraperitoneally with 1000g/L herba epimodiu extract（0.01 mL/g）, 15 minutes later, intraperitoneally injected with 0.01mL/g subthreshold dose of pentobarbital sodium （0.25%）, and then observe the disappearance time of righting reflex of the mouse. MAIN OUTCOME MEASURES： Frequency of spontaneous activity of the mice; sleeping time of the mouse with suprathreshold and subthreshold dose of pentobarbit

关 键 词：淫羊藿/药理学 行为 动物/药物作用 睡眠/生理学 

分 类 号：R338.63[医药卫生—人体生理学]