补阳还五汤的抗氧化作用  

Antioxidant effect of Buyanghuanwutang

作　　者：邢三丽[1] 李振华[1] 孙晋浩[1] 刘岳鹏[1] 暴丽华[1] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012

出　　处：《解剖学杂志》2005年第5期529-531,F0002,共4页Chinese Journal of Anatomy

基　　金：山东省自然科学基金(Y2000C14);山东省优秀中青年科学家科研奖励基金(2004BS02012)

摘　　要：目的:探讨复方中药制剂补阳还五汤的抗氧化机制。方法:用水煎醇沉法制备补阳还五汤提取液,从新生鼠坐骨神经分离纯化雪旺细胞,建立过氧化氢(H2O2)损伤模型。将培养细胞分为补阳还五汤处理组、氧化损伤组及正常组和空白对照组,培养12 h时,检测丙二醛(MDA)的含量,用免疫组化方法检测Caspase-3的表达,用RT-PCR技术检测胶质细胞源性神经营养因子(GDNF)的表达。结果:与氧化损伤组相比,补阳还五汤处理组的MDA含量明显减弱,Caspase-3呈弱阳性表达,GDNF mRNA的表达量减少不明显。结论:补阳还五汤具有良好的抗过氧化损伤效果,对H2O2引起雪旺细胞的损伤具有保护作用。Objective： To explore the antioxidant mechanism of Buyanghuanwutang （BYHWT）. Methods：Schwarm cells were purified from the sciatic nerve of neonatal rats and were divided into 3 groups： H2O2 group, BYHWT group and normal group. Malondialdehyde （MDA） content in Schwann cells was determined by biochemical analyzer after 12 h, the expression of Caspase-3 was detected by immunohistochemistry, and the expression of GDNF rnRNA was detected by RT-PCR. Resuits： Compared with H2O2 group, intracellular MDA content in BYHWT group was significantly lower and expression of GDNF mRNA kept at a higher level （P〈0.05）. Conclusion： BYHWT has a good antioxidant effect and can be used to protect the Schwarm cell from damage caused by H2O2.

关 键 词：补阳还五汤 雪旺细胞 氧化损伤 丙二醛 Caspase-3胶质细胞源性神经营养因子 

分 类 号：R285[医药卫生—中药学]