大鼠听皮质NMDA受体亚单位NR2B蛋白质的年龄-依赖性表达  

Age-dependent expression of NMDA receptor subunit NR2B protein in rat auditory cortex

作　　者：张凌[1] 吴秀梅[1] 徐凤[1] 许兢宏[1] 孙心德[1] 

机构地区：[1]华东师范大学生命科学学院脑科学研究中心,上海200062

出　　处：《解剖学杂志》2005年第5期566-569,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30170313);博士点基金(20020269005);上海市神经科学重点学科项目

摘　　要：目的:研究SD大鼠生后发育过程中,听皮质神经元NMDA受体亚单位NR2B蛋白质的表达规律。方法:采用免疫组织化学反应和蛋白质印迹技术,分别检测生后1、2、3周和成年动物听皮质神经元NMDA受体亚单位NR2B蛋白质的表达。结果:NR2B阳性神经元在生后第1周密度最高,随着生后周龄增长,NR2B阳性神经元密度递减,3周龄后降至成年动物的低表达水平。结论:大鼠生后发育过程中,听皮质NR2B亚单位蛋白质呈现年龄-依赖性表达,此结果与mRNA水平上的表达趋势一致。Objective.. To study the age-dependent expression of NMDA receptor subunit NR2B protein during postnatal develop ment in the rat auditory cortex. Methods： Western blotting and immunocytochemistry techniques were used to examine NR2B protein expression on postnatal week （PNW） 1,2,3 and in adult rats. Results： The density of NR2B-positive neurons was detected in the auditory cortex during development. The protein expression of NR2B was the highest on PNW 1, and then began to decline by PNW 3 to a low level as in adults. Conclusion： Age-dependent expression of NR2B protein in rats during postnatal period is consistant with the mRNA levels, which may help to understand the molecular mechanism of developmental plasticity of auditory cortex.

关 键 词：大鼠 听皮质 免疫组织化学 NMDA受体 NR2B亚单位 

分 类 号：Q593.2[生物学—生物化学]