增殖细胞核抗原在小鼠生后肾脏发育中的表达  

Expression of proliferating cell nuclear antigen in mouse kidney during postnatal development

作　　者：李晓明[1] 郭敏[1] 

机构地区：[1]锦州医学院组织学与胚胎学教研室,锦州121001

出　　处：《解剖学杂志》2005年第5期577-579,F0003,共4页Chinese Journal of Anatomy

基　　金：辽宁省自然科学基金(20032140)

摘　　要：目的:观察小鼠出生后肾脏发育过程中增殖细胞核抗原(PCNA)的表达特征,探讨出生后小鼠肾脏发育过程中细胞增殖的规律.方法:应用免疫组织化学技术检测小鼠出生后1、3、7、14、21、28和70d肾脏PCNA的表达.结果:小鼠出生后1～70d,皮质中的生肾区、肾小体、肾小管、髓放线以及髓质中的肾小管和集合管PCNA阳性细胞的表达具有一定的规律,早期PCNA阳性表达丰富,随着肾脏发育逐渐成熟而表达减弱直至消失.在70d成年小鼠肾脏中,没有检测到PCNA阳性细胞.结论:出生后小鼠肾脏皮质中的生肾区、肾小体、肾小管、髓放线以及髓质中小管的细胞增殖规律是由高逐渐降低的,直至成年完全停止.Objective： To observe the expressin of proliferating cell nuclear antigen （PCNA） and investigate cell proliferation in the mouse kidney during postnatal development. Methods： The expression of PCNA was examined in mouse kidney at 1,3,7,14,21, 28 and 70 d after birth by immunohistochemical technique. Results： Cell proliferation was prominent in nephrogenic, glomeruli, tubules, medullary rays and medulla during 1-70 days after birth. PCNA-positive cells were abundant at the early stage, decreased gradually following the renal developmental stage and eventually stopped. PCNA positive cells were not found in adult kidney. Conclusion： Proliferative activity decreases gradually in subcapsular zone, glomeruli, tubules, medullary rays and medulla during postnatal development.

关 键 词：小鼠 肾脏 细胞增殖 免疫组织化学 

分 类 号：R392[医药卫生—免疫学]