猫腰髓白质年龄相关的形态学变化  

Age-related Morphological Changes in the White Matter of Cat Lumbar Spinal Cord

作　　者：朱再满[1] 张长征[1] 罗勋[1] 孙庆艳[1] 梅斌[1] 华田苗[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《Zoological Research》2005年第5期527-533,共7页动物学研究（英文）

基　　金：安徽省自然科学基金(98241716);安徽省教育厅科研基金(2003kj152);安徽省重要生物资源保护与利用研究重点实验室科研基金;安徽师范大学青年基金(2004xqn09)

摘　　要：以青年成年猫(1-3龄,2-2．5 kg)和老年猫(12龄,3-3．5kg)L6段脊髓白质为研究对象,用 神经丝蛋白(NF)免疫染色显示神经纤维,用改良的Holzer结晶紫染色显示所有胶质细胞并用成年动物Golgi 法显示其形态,用胶质纤维酸性蛋白(GFAP)免疫染色显示星形胶质细胞。光镜下对青年猫与老年猫腰髓白质 中神经纤维和胶质细胞进行形态学观察和定量研究。与青年猫相比,老年猫腰髓白质中的神经纤维密度显著下 降(P<O．01),NF免疫反应阳性较弱;胶质细胞密度显著增加(P<0．01);GFAP免疫阳性细胞密度显著增 加(P<0．01),GFAP阳性细胞阳性反应较强,胞体明显膨大,突起稠密粗大。表明猫在衰老过程中,脊髓白 质中有神经纤维丢失及胶质细胞增生现象。提示动物在衰老过程中脊髓白质的神经纤维丢失可能是脊髓传导功 能衰退的直接原因;白质中胶质细胞的增多与活动增强可能足对神经纤维丢失的补偿性增生,并可延缓脊髓的 衰老。In order to investigate age-related morphological changes in the white matter of the lumbar spinal cord in young adult and old cats, several techniques were used. （1） lmmunostaining of neurofilaments （NF） was used to identify nerve fibres; （2） modified Holzer crystal violet to show all the glial cells; （3） immunoreaction of glial fibrillary acid protein （GFAP） to exhibit the astrocytes; （4） the Golgi method to display the morphology of glial cells and astrocytes in the white matter. Under the microscope, the fibres and glial cells were observed and quantitatively studied. Compared with young cats, old cats showed significantly lower density of nerve fibres （P 〈 0.01 ）and weaker staining of the NF immunoreactivity. However, the density of glial cells and astrocytes（P 〈0.01 ）in old cats is significantly greater than in young adult cats. In addition, astrocytes in old cats were more hypertrophic, with stronger immunoreactivity of GFAP substance than in young ones. This indicates that there is a loss of nerve fibres accompanied by an obvious glial hyperplasia in the white matter of the aging cat spinal cord. The above results suggest that the loss of nerve fibres in the white matter of old cats may be an important factor that underlies the functional decline of signal transmission and processing in the spinal cord during ageing. The activity enhancement of glial cells in old cats may provide a protective effect on surviving nerve fibers from further degradation during senescence.

关 键 词：猫 脊髓 神经纤维 胶质细胞 星形胶质细胞 年龄相关变化 

分 类 号：Q954.67[生物学—动物学]