早期应用激活素A对新生大鼠缺氧缺血性脑损伤的预防作用  

Protective effects of activin A on brain tissue of neonatal rats with hypoxic ischemic brain damage

作　　者：安丽[1] 孙若鹏[2] 刘怡平[1] 马建华[1] 

机构地区：[1]济南市中心医院小儿内科,山东济南250013 [2]山东大学齐鲁医院小儿内科,山东济南250012

出　　处：《山东大学学报（医学版）》2005年第10期902-905,共4页Journal of Shandong University:Health Sciences

基　　金：山东省优秀中青年科学家科研奖励基金资助项目(2004BS03011)。

摘　　要：目的:研究外源性激活素A(activinA,ACTA)对新生大鼠缺氧缺血性脑损伤(hypoxicischemicbraindamage,HIBD)的预防作用及其机制。方法:随机将60只新生7日龄Wistar大鼠分为6组,每组10只,即:正常对照组、HIBD模型组、假手术组及治疗组I、II、III(分别腹腔注射高、中、低剂量ACTA)。缺氧缺血(HI)后,治疗组即刻腹腔注射ACTA,其他各组均腹腔注射等量生理盐水。各组于HI结束后不同时间点(0、2、6、24、48h)分批采集标本,观察ACTA对HIBD模型鼠脑重的增长、左右脑重量差值、脑组织病理学变化及脑组织超微结构变化的影响;应用流式细胞仪分析细胞凋亡,观察ACTA对HIBD后脑细胞凋亡的影响。结果:造模后各时间点,各治疗组脑组织淤血及水肿程度较模型组明显减轻,脑重增加值及左右脑重差值的均值与模型组比较均有显著性差异(1.21±0.07vs1.29±0.11,P<0.05;0.059±0.036vs0.092±0.063,P<0.01);光镜及电镜下组织细胞结构较模型组均有不同程度的修复;治疗组脑组织细胞凋亡均值明显低于模型组(4.87±0.13vs7.46±0.12,P<0.05);治疗组以上各项指标的均值与正常对照组及假手术组比较、各治疗组之间两两比较,差异均无统计学意义(P>0.05)。结论:外源性ACTA具有抑制新生大鼠HIBD后脑组织水肿及脑细胞凋亡,从而减轻脑损伤的作用。Objective： To investigate the preventive effects and possible mechanism of exogenous activin A （ACT A） on brain tissue in neonatal rats with hypoxic ischemic brain damage （HIBD）. Methods： Totally 60 seven-day-old Wistar rats were divided into 6 groups （10 for each） randomly as below： normal control group, HIBD model group, fake surgery group and treatment groups Ⅰ, Ⅱ, and Ⅲ （respectively injected into the belly cavity with different dosages of ACT A）. The treatment groups were given ACT A instantly after hypoxic-ischemia （HI）, and other groups were given the same volume of physiological saline. The specimens brain tissues alleviated obviously than those in model group on different time points after HI. The brain weight and the difference between left and right brain weight decreased obviously than those in the model group （1.21±0.07 vs 1.29±0.11, P〈0.05; 0.059±0.036 vs 0.092±0.063, P〈0.01）. Under light microscope and electron microscope, plerosis of brain tissue in pathologic structure and ultrastructure was found in different degrees in treatment groups. The mean value of apoptosis in model group was obviously higher than that in treatment groups （7.46＋0.12 vs 4.87＋0.13, P〈0.05）. Conclusion： The exogenous ACT A can protect the brain tissue by reducing the cerebral edema and depressing apoptosis in neonatal rat with H1BD.

关 键 词：激活素A 大鼠 新生 脑缺氧 脑缺血 细胞凋亡 

分 类 号：R512.3[医药卫生—内科学]