中药颤复宁血清对6-羟基多巴诱导的PC12细胞凋亡的抑制作用  

Inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine

作　　者：孟海伟[1] 刘树伟[1] 许继平[2] 冯蕾[1] 胡坚莉[1] 侯中煜[1] 程葆华[1] 

机构地区：[1]山东大学医学院解剖学教研室,山东济南250012 [2]山东大学第二医院神经内科,山东济南250033

出　　处：《山东大学学报（医学版）》2005年第10期916-919,共4页Journal of Shandong University:Health Sciences

摘　　要：目的:探讨中药颤复宁血清对6羟基多巴(6OHDA)诱导的大鼠嗜铬细胞瘤细胞(PC12)凋亡的抑制作用。方法:用血清药理学方法制备中药血清,加到经6OHDA处理的PC12细胞培养液中,用四甲基偶氮唑蓝(MTT)法检测细胞存活率,并用免疫组织化学染色方法和图像分析检测酪氨酸羟化酶(TH)含量及半胱氨酸蛋白酶3(Caspase3)阳性凋亡细胞数。结果:颤复宁中药血清能有效地提高PC12细胞的存活率,对6OHDA诱导的凋亡有明显抑制作用。与6OHDA损伤组比较,颤复宁血清组TH阳性细胞平均吸光度增高(P<0.01),Caspase3阳性细胞数明显降低(P<0.01)。结论:中药颤复宁血清对PC12细胞具有直接的神经营养作用,能提高细胞存活率,且对6OHDA诱导的细胞凋亡具有抑制作用。Objective： To study the inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine （6-OHDA）. Methods： Serum pharmacological method was used to make chanfuning serum. 6-OHDA and ehanfuning serum were added into the medium of PC12 cells separately. MTT method was used to assay the cell＇s viability, and tyrosine hydroxylase （TH） and caspase-3 immunocytochemistry staining were used to evaluate the content of TH and count the number of apoptosis cells. Resuits： PC12 cell＇s viability and calibrated optic density of TH positive cells enhanced （P〈0.01）, and the number of caspase-3 positive cells decreased （P〈0.01） significantly in chanfuning serum treated group compared with 6-OHDA treated group. Conclusion： Chanfuning serum may have direct nutritious effect, protect PC12 cells from injury induced by 6-OHDA, and produce anti-apoptosis effect to some extent.

关 键 词：6-羟基多巴 PC12细胞 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 帕金森病 

分 类 号：R285[医药卫生—中药学]